Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway
ABSTRACT: Sarcopenia is the age-related loss of muscle and fiber number and decreased regenerative capacity with increased abundance of reactive oxygen species levels and electron transport chain abnormalities. The aim of this study was to investigate the antisarcopenia effect of MFG-E8 in alleviati...
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Elsevier
2025-02-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224012414 |
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| author | Kaifang Guan Xiaolin Liu Weihong Lu Yuhao Mao Yirong Mao Ying Ma Rongchu Wang Qiming Li |
| author_facet | Kaifang Guan Xiaolin Liu Weihong Lu Yuhao Mao Yirong Mao Ying Ma Rongchu Wang Qiming Li |
| author_sort | Kaifang Guan |
| collection | DOAJ |
| description | ABSTRACT: Sarcopenia is the age-related loss of muscle and fiber number and decreased regenerative capacity with increased abundance of reactive oxygen species levels and electron transport chain abnormalities. The aim of this study was to investigate the antisarcopenia effect of MFG-E8 in alleviating skeletal muscle dysfunction induced by D-galactose, and reveal the mechanism promoting myoblast cell proliferation and mediating the cell cycle. This in vivo experiment showed that MFG-E8 can improve the antioxidant status and increase soleus muscle mass (35.61%) and fiber diameter (39.72%) in the aging rats. The western blot assay preliminarily proved that increased ERK phosphorylation determines the repairment of injured skeletal muscle, but not JNK and p38. In vitro experiments further verified that MFG-E8 can increase the number of mitochondria, cell vitality, cell density, and reduce apoptosis rate. Flow cytometry and quantitative real-time PCR proved that MFG-E8 promoted cell proliferation by upregulating mRNA expression of cyclin D1, cyclin E1, CDK, and downregulating mRNA expression of p21 and p27, thereby increasing the S and G2/M phase and decreasing the G0/G1 phase. Molecular level further proved that MFG-E8 mediated cell cycle and promoted cell proliferation by activating the MAPK/ERK signaling pathway. |
| format | Article |
| id | doaj-art-823bd820fcbe41c3b2f71043f4cfd4fa |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-823bd820fcbe41c3b2f71043f4cfd4fa2025-01-23T05:25:09ZengElsevierJournal of Dairy Science0022-03022025-02-01108211821197Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathwayKaifang Guan0Xiaolin Liu1Weihong Lu2Yuhao Mao3Yirong Mao4Ying Ma5Rongchu Wang6Qiming Li7School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, Heilongjiang, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, Heilongjiang, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, Heilongjiang, ChinaCollege of Architectural Science and Engineering, Yangzhou University, Jiangsu 225000, ChinaSchool of Science, Shanghai Maritime University, Shanghai 200135, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, Heilongjiang, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, Heilongjiang, China; Corresponding authorsDairy Nutrition and Function, Key Laboratory of Sichuan Province, New Hope Dairy Co. Ltd., Chengdu, Sichuan 610023, China; Chengdu Molecular Power Biotechnology Co. Ltd., Chengdu, Sichuan 611732, China; Corresponding authorsABSTRACT: Sarcopenia is the age-related loss of muscle and fiber number and decreased regenerative capacity with increased abundance of reactive oxygen species levels and electron transport chain abnormalities. The aim of this study was to investigate the antisarcopenia effect of MFG-E8 in alleviating skeletal muscle dysfunction induced by D-galactose, and reveal the mechanism promoting myoblast cell proliferation and mediating the cell cycle. This in vivo experiment showed that MFG-E8 can improve the antioxidant status and increase soleus muscle mass (35.61%) and fiber diameter (39.72%) in the aging rats. The western blot assay preliminarily proved that increased ERK phosphorylation determines the repairment of injured skeletal muscle, but not JNK and p38. In vitro experiments further verified that MFG-E8 can increase the number of mitochondria, cell vitality, cell density, and reduce apoptosis rate. Flow cytometry and quantitative real-time PCR proved that MFG-E8 promoted cell proliferation by upregulating mRNA expression of cyclin D1, cyclin E1, CDK, and downregulating mRNA expression of p21 and p27, thereby increasing the S and G2/M phase and decreasing the G0/G1 phase. Molecular level further proved that MFG-E8 mediated cell cycle and promoted cell proliferation by activating the MAPK/ERK signaling pathway.http://www.sciencedirect.com/science/article/pii/S0022030224012414MFG-E8skeletal muscle atrophymitochondriaERK signaling pathwaycell cycle |
| spellingShingle | Kaifang Guan Xiaolin Liu Weihong Lu Yuhao Mao Yirong Mao Ying Ma Rongchu Wang Qiming Li Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway Journal of Dairy Science MFG-E8 skeletal muscle atrophy mitochondria ERK signaling pathway cell cycle |
| title | Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway |
| title_full | Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway |
| title_fullStr | Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway |
| title_full_unstemmed | Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway |
| title_short | Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway |
| title_sort | bioactive milk derived nutrient mfg e8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the mapk erk signaling pathway |
| topic | MFG-E8 skeletal muscle atrophy mitochondria ERK signaling pathway cell cycle |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224012414 |
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