Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso
The emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses...
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Wiley
2023-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/4813225 |
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| author | Boukaré Kaboré Henri S. Ouédraogo Oumarou Zongo Ganamé Abasse Ouédraogo François Tapsoba Sanogo Bougma Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Idrissa Sanou Aly Savadogo |
| author_facet | Boukaré Kaboré Henri S. Ouédraogo Oumarou Zongo Ganamé Abasse Ouédraogo François Tapsoba Sanogo Bougma Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Idrissa Sanou Aly Savadogo |
| author_sort | Boukaré Kaboré |
| collection | DOAJ |
| description | The emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. This study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at a biomedical analysis laboratory. A total of fifty-three unduplicated E. coli strains isolated from patient samples with a multidrug-resistant (MDR) profile were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. This study allowed us to identify fifteen strains carrying resistance genes among the fifty-three E. coli strains. All fifteen strains produced the metallo-β-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes were detected in three strains and VIM was identified in two strains of E. coli. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Thus, NDM and VIM are the main carbapenemases detected in the strains in our study. |
| format | Article |
| id | doaj-art-820c7f4b814643e0af3b8f09eca5062c |
| institution | OA Journals |
| issn | 1687-9198 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-820c7f4b814643e0af3b8f09eca5062c2025-08-20T02:09:05ZengWileyInternational Journal of Microbiology1687-91982023-01-01202310.1155/2023/4813225Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina FasoBoukaré Kaboré0Henri S. Ouédraogo1Oumarou Zongo2Ganamé Abasse Ouédraogo3François Tapsoba4Sanogo Bougma5Koudbi Jacob Zongo6Boukaré Zeba7Yves Traoré8Idrissa Sanou9Aly Savadogo10Department of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyDepartment of Biochemistry and MicrobiologyUFR Health SciencesDepartment of Biochemistry and MicrobiologyThe emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. This study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at a biomedical analysis laboratory. A total of fifty-three unduplicated E. coli strains isolated from patient samples with a multidrug-resistant (MDR) profile were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. This study allowed us to identify fifteen strains carrying resistance genes among the fifty-three E. coli strains. All fifteen strains produced the metallo-β-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes were detected in three strains and VIM was identified in two strains of E. coli. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Thus, NDM and VIM are the main carbapenemases detected in the strains in our study.http://dx.doi.org/10.1155/2023/4813225 |
| spellingShingle | Boukaré Kaboré Henri S. Ouédraogo Oumarou Zongo Ganamé Abasse Ouédraogo François Tapsoba Sanogo Bougma Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Idrissa Sanou Aly Savadogo Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso International Journal of Microbiology |
| title | Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso |
| title_full | Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso |
| title_fullStr | Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso |
| title_full_unstemmed | Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso |
| title_short | Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso |
| title_sort | emergence of new delhi metallo β lactamase ndm genes detected from clinical strains of escherichia coli isolated in ouagadougou burkina faso |
| url | http://dx.doi.org/10.1155/2023/4813225 |
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