Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i>
Wild and domesticated emmer (ВВАА, 2n = 28) are of significant interest for expanding the genetic diversity of common wheat as sources of a high protein and microelement grain content, resistance to many biotic and abiotic factors. Particular interest in these species is also determined by their clo...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2023-11-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3926 |
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| author | О. A. Orlovskaya I. N. Leonova L. A. Solovey N. I. Dubovets |
| author_facet | О. A. Orlovskaya I. N. Leonova L. A. Solovey N. I. Dubovets |
| author_sort | О. A. Orlovskaya |
| collection | DOAJ |
| description | Wild and domesticated emmer (ВВАА, 2n = 28) are of significant interest for expanding the genetic diversity of common wheat as sources of a high protein and microelement grain content, resistance to many biotic and abiotic factors. Particular interest in these species is also determined by their close relationship with Triticum aestivum L., which facilitates interspecific hybridization. The objective of this work was to analyze the nature of alien introgressions in hybrid lines from crossing common wheat varieties with T. dicoccoides and T. dicoccum, and to assess the effect of their genome fragments on the cytological stability of introgression lines. A C-banding technique and genotyping with SNP and SSR markers were used to determine localization and length of introgression fragments. Assessment of cytological stability was carried out on the basis of chromosome behavior in microsporogenesis. A molecular cytogenetic analysis of introgression wheat lines indicated that the inclusion of the genetic material of wild and domesticated emmer was carried out mainly in the form of whole arms or large fragments in the chromosomes of the B genome and less extended inserts in the A genome. At the same time, the highest frequency of introgressions of the emmer genome was observed in chromosomes 1A, 1B, 2B, and 3B. The analysis of the final stage of meiosis showed a high level of cytological stability in the vast majority of introgression wheat lines (meiotic index was 83.0–99.0 %), which ensures the formation of functional gametes in an amount sufficient for successful reproduction. These lines are of interest for the selection of promising material with agronomically valuable traits and their subsequent inclusion in the breeding process. |
| format | Article |
| id | doaj-art-8205e0400dba40fc871b002c7991c968 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-8205e0400dba40fc871b002c7991c9682025-02-01T09:58:12ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592023-11-0127655356410.18699/VJGB-23-671383Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i>О. A. Orlovskaya0I. N. Leonova1L. A. Solovey2N. I. Dubovets3Institute of Genetics and Cytology of the National Academy of Sciences of BelarusInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State Agricultural UniversityInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusWild and domesticated emmer (ВВАА, 2n = 28) are of significant interest for expanding the genetic diversity of common wheat as sources of a high protein and microelement grain content, resistance to many biotic and abiotic factors. Particular interest in these species is also determined by their close relationship with Triticum aestivum L., which facilitates interspecific hybridization. The objective of this work was to analyze the nature of alien introgressions in hybrid lines from crossing common wheat varieties with T. dicoccoides and T. dicoccum, and to assess the effect of their genome fragments on the cytological stability of introgression lines. A C-banding technique and genotyping with SNP and SSR markers were used to determine localization and length of introgression fragments. Assessment of cytological stability was carried out on the basis of chromosome behavior in microsporogenesis. A molecular cytogenetic analysis of introgression wheat lines indicated that the inclusion of the genetic material of wild and domesticated emmer was carried out mainly in the form of whole arms or large fragments in the chromosomes of the B genome and less extended inserts in the A genome. At the same time, the highest frequency of introgressions of the emmer genome was observed in chromosomes 1A, 1B, 2B, and 3B. The analysis of the final stage of meiosis showed a high level of cytological stability in the vast majority of introgression wheat lines (meiotic index was 83.0–99.0 %), which ensures the formation of functional gametes in an amount sufficient for successful reproduction. These lines are of interest for the selection of promising material with agronomically valuable traits and their subsequent inclusion in the breeding process.https://vavilov.elpub.ru/jour/article/view/3926common wheattriticum aestivumt. dicoccoidest. dicoccumintrogression linesc-bandingssr analysissnp analysismicrosporogenesiscytological stability |
| spellingShingle | О. A. Orlovskaya I. N. Leonova L. A. Solovey N. I. Dubovets Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> Вавиловский журнал генетики и селекции common wheat triticum aestivum t. dicoccoides t. dicoccum introgression lines c-banding ssr analysis snp analysis microsporogenesis cytological stability |
| title | Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> |
| title_full | Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> |
| title_fullStr | Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> |
| title_full_unstemmed | Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> |
| title_short | Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> |
| title_sort | molecular cytological analysis of alien introgressions in common wheat lines created by crossing of i triticum aestivum i with i t dicoccoides i and i t dicoccum i |
| topic | common wheat triticum aestivum t. dicoccoides t. dicoccum introgression lines c-banding ssr analysis snp analysis microsporogenesis cytological stability |
| url | https://vavilov.elpub.ru/jour/article/view/3926 |
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