Pediatric kidney transplant recipients are at an increased risk for dysbiosis
IntroductionThis study aimed to compare the gut microbiota composition in pediatric kidney transplant (KTx) recipient with that of healthy children.MethodsThis cross-sectional observational study included 30 pediatric KTx recipients aged between 7 and 21 years and 25 healthy children. The gut microb...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1499813/full |
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| author | Gizem Yılmaz Seha Saygılı Ayşe Ağbaş Esra Karabağ Yılmaz Ahmet Variş Nur Canpolat |
| author_facet | Gizem Yılmaz Seha Saygılı Ayşe Ağbaş Esra Karabağ Yılmaz Ahmet Variş Nur Canpolat |
| author_sort | Gizem Yılmaz |
| collection | DOAJ |
| description | IntroductionThis study aimed to compare the gut microbiota composition in pediatric kidney transplant (KTx) recipient with that of healthy children.MethodsThis cross-sectional observational study included 30 pediatric KTx recipients aged between 7 and 21 years and 25 healthy children. The gut microbiota was assessed using 16S rRNA gene sequencing, with alpha and beta diversity, as well as all statistical analyses, conducted using the Phyloseq library in the R programming language. Taxonomic profiles were evaluated with QIIME2, and differences in gut microbiota profiles were compared using linear discriminant analysis effect size (LEFSe) with an LDA threshold of >2 and p < 0.05.ResultsNo significant differences were found in alpha and beta diversity between the KTx recipients and healthy controls. However, KTx recipients exhibited significant alterations in microbiota composition, including higher relative abundances of Verrucomicrobiota at the phylum level, and Akkermansia and Neisseria at the genus level (p < 0.05 for all). Conversely, there was a decrease in bacterial genera belonging to the phylum Firmicutes. In addition, KTx recipients with a history of frequent urinary tract infections, diarrhea and reduced GFR showed significant increases in bacterial abundance (p < 0.05 for all).DiscussionPediatric KTx recipients demonstrated significant alterarions in gut microbiota composition, indicating dysbiosis. Further studies are needed to elucidate the cause-and-effect relationships of these changes and their impact on clinical consequencies and long-term prognosis. |
| format | Article |
| id | doaj-art-81eba2af4df141b1a82a593a676cb797 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-81eba2af4df141b1a82a593a676cb7972025-01-30T16:02:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14998131499813Pediatric kidney transplant recipients are at an increased risk for dysbiosisGizem Yılmaz0Seha Saygılı1Ayşe Ağbaş2Esra Karabağ Yılmaz3Ahmet Variş4Nur Canpolat5Department of Pediatrics, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, TürkiyeDepartment of Pediatric Nephrology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, TürkiyeDepartment of Pediatric Nephrology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, TürkiyeDepartment of Pediatric Nephrology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, TürkiyeDiagen Biotechnology, Ankara, TürkiyeDepartment of Pediatric Nephrology, Cerrahpasa Faculty of Medicine, Istanbul University - Cerrahpasa, Istanbul, TürkiyeIntroductionThis study aimed to compare the gut microbiota composition in pediatric kidney transplant (KTx) recipient with that of healthy children.MethodsThis cross-sectional observational study included 30 pediatric KTx recipients aged between 7 and 21 years and 25 healthy children. The gut microbiota was assessed using 16S rRNA gene sequencing, with alpha and beta diversity, as well as all statistical analyses, conducted using the Phyloseq library in the R programming language. Taxonomic profiles were evaluated with QIIME2, and differences in gut microbiota profiles were compared using linear discriminant analysis effect size (LEFSe) with an LDA threshold of >2 and p < 0.05.ResultsNo significant differences were found in alpha and beta diversity between the KTx recipients and healthy controls. However, KTx recipients exhibited significant alterations in microbiota composition, including higher relative abundances of Verrucomicrobiota at the phylum level, and Akkermansia and Neisseria at the genus level (p < 0.05 for all). Conversely, there was a decrease in bacterial genera belonging to the phylum Firmicutes. In addition, KTx recipients with a history of frequent urinary tract infections, diarrhea and reduced GFR showed significant increases in bacterial abundance (p < 0.05 for all).DiscussionPediatric KTx recipients demonstrated significant alterarions in gut microbiota composition, indicating dysbiosis. Further studies are needed to elucidate the cause-and-effect relationships of these changes and their impact on clinical consequencies and long-term prognosis.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1499813/fullchildrendysbiosisgutintestinekidney transplantationmicrobiota |
| spellingShingle | Gizem Yılmaz Seha Saygılı Ayşe Ağbaş Esra Karabağ Yılmaz Ahmet Variş Nur Canpolat Pediatric kidney transplant recipients are at an increased risk for dysbiosis Frontiers in Microbiology children dysbiosis gut intestine kidney transplantation microbiota |
| title | Pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| title_full | Pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| title_fullStr | Pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| title_full_unstemmed | Pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| title_short | Pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| title_sort | pediatric kidney transplant recipients are at an increased risk for dysbiosis |
| topic | children dysbiosis gut intestine kidney transplantation microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1499813/full |
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