Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages
The important factors of poor survival of gastric cancer (GC) are relapse and metastasis. For further elucidation of the mechanism, a culture system mimicking the microenvironment of the tumor in humans was needed. We established a model of microencapsulated SGC7901 human GC cells and evaluated the...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2018/3767482 |
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| author | Jin-Ming Zhu Xiu-Lian Quan Shi-Chao Han Xue-Jun Fan He-Ming Li Shan-Shan Liang Xi Chen Ruo-Yu Wang Xue-Ning Ji |
| author_facet | Jin-Ming Zhu Xiu-Lian Quan Shi-Chao Han Xue-Jun Fan He-Ming Li Shan-Shan Liang Xi Chen Ruo-Yu Wang Xue-Ning Ji |
| author_sort | Jin-Ming Zhu |
| collection | DOAJ |
| description | The important factors of poor survival of gastric cancer (GC) are relapse and metastasis. For further elucidation of the mechanism, a culture system mimicking the microenvironment of the tumor in humans was needed. We established a model of microencapsulated SGC7901 human GC cells and evaluated the effects of coculturing spheres with tumor-associated macrophages (TAMs). SGC7901 cells were encapsulated in alginate-polylysine-sodium alginate (APA) microcapsules using an electrostatic droplet generator. MTT assays showed that the numbers of microencapsulated cells were the highest after culturing for 14 days. Metabolic curves showed consumption of glucose and production of lactic acid by day 20. Immunocytochemistry confirmed that Proliferating Cell Nuclear Antigen (PCNA) and Vascular Endothelial Growth Factor (VEGF) were expressed in microencapsulated SGC7901 cells on days 7 and 14. The expression of PCNA was observed outside spheroids; however, VEGF was found in the entire spheroids. PCNA and VEGF were increased after being cocultured with TAMs. Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were detected in the supernatant of microencapsulated cells cocultured with TAMs but not in microencapsulated cells. Our study confirms the successful establishment of the microencapsulated GC cells. TAMs can promote PCNA, VEGF, MMP-2, and MMP-9 expressions of the GC cells. |
| format | Article |
| id | doaj-art-81cba55c24fd4daab69fe9cbc5d81bca |
| institution | Kabale University |
| issn | 2291-2789 2291-2797 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-81cba55c24fd4daab69fe9cbc5d81bca2025-02-03T01:12:25ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972018-01-01201810.1155/2018/37674823767482Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated MacrophagesJin-Ming Zhu0Xiu-Lian Quan1Shi-Chao Han2Xue-Jun Fan3He-Ming Li4Shan-Shan Liang5Xi Chen6Ruo-Yu Wang7Xue-Ning Ji8Department of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Gynaecology and Obstetrics, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, ChinaDepartment of Oncology, Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaDepartment of Oncology, Zhongshan Hospital, Dalian University, Dalian, Liaoning, ChinaThe important factors of poor survival of gastric cancer (GC) are relapse and metastasis. For further elucidation of the mechanism, a culture system mimicking the microenvironment of the tumor in humans was needed. We established a model of microencapsulated SGC7901 human GC cells and evaluated the effects of coculturing spheres with tumor-associated macrophages (TAMs). SGC7901 cells were encapsulated in alginate-polylysine-sodium alginate (APA) microcapsules using an electrostatic droplet generator. MTT assays showed that the numbers of microencapsulated cells were the highest after culturing for 14 days. Metabolic curves showed consumption of glucose and production of lactic acid by day 20. Immunocytochemistry confirmed that Proliferating Cell Nuclear Antigen (PCNA) and Vascular Endothelial Growth Factor (VEGF) were expressed in microencapsulated SGC7901 cells on days 7 and 14. The expression of PCNA was observed outside spheroids; however, VEGF was found in the entire spheroids. PCNA and VEGF were increased after being cocultured with TAMs. Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were detected in the supernatant of microencapsulated cells cocultured with TAMs but not in microencapsulated cells. Our study confirms the successful establishment of the microencapsulated GC cells. TAMs can promote PCNA, VEGF, MMP-2, and MMP-9 expressions of the GC cells.http://dx.doi.org/10.1155/2018/3767482 |
| spellingShingle | Jin-Ming Zhu Xiu-Lian Quan Shi-Chao Han Xue-Jun Fan He-Ming Li Shan-Shan Liang Xi Chen Ruo-Yu Wang Xue-Ning Ji Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages Canadian Journal of Gastroenterology and Hepatology |
| title | Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages |
| title_full | Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages |
| title_fullStr | Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages |
| title_full_unstemmed | Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages |
| title_short | Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages |
| title_sort | establishment of a model of microencapsulated sgc7901 human gastric carcinoma cells cocultured with tumor associated macrophages |
| url | http://dx.doi.org/10.1155/2018/3767482 |
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