Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain)
A feeding trial was conducted to evaluate the enzyme-hydrolyzed marine proteins (EMP, comprising fish protein peptides, enzymatic squid protein, and seaweed polysaccharides) supplementation on the growth performance, protein metabolism and immune responses of mud crabs (Scylla paramamosain). Four is...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003904 |
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| author | Tiantian Xu Xiaoyue Li Min Jin Zheng Tang Xishuai Cui Yinzhao Zhang Feng Tang Qicun Zhou |
| author_facet | Tiantian Xu Xiaoyue Li Min Jin Zheng Tang Xishuai Cui Yinzhao Zhang Feng Tang Qicun Zhou |
| author_sort | Tiantian Xu |
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| description | A feeding trial was conducted to evaluate the enzyme-hydrolyzed marine proteins (EMP, comprising fish protein peptides, enzymatic squid protein, and seaweed polysaccharides) supplementation on the growth performance, protein metabolism and immune responses of mud crabs (Scylla paramamosain). Four isonitrogenous and isolipidic diets were prepared with different treatments: a normal control (NC) containing 30 % FM, a positive control (PC) with 30 % FM supplemented with 2 % EMP, an experimental diet with 28 % FM and 1 % EMP (EMP1), and another experimental diet with 26 % FM and 2 % EMP (EMP2). A total of 120 healthy mud crabs were randomly assigned to individual crab apartments, with three replicates per treatment (10 crabs per replicate). Results showed that crabs given the EMP2 diet exhibited the highest PWG and SGR (P < 0.05). Crabs on the NC diet showed substantially decreased pepsin and amylase activities in the hepatopancreas and stomach compared to other diets (P < 0.05). Dietary EMP supplementation significantly upregulated the expression of molting-related genes (ecr, met, chi, e75, chh, and famet) in the hepatopancreas, protein metabolism-related genes (akt, rheb, tor, pi3k, irs, s6k, eif4e, 4ebp1, and 4ebp2) in both the hepatopancreas and intestines, and immune-related genes (lysozyme, proPO, toll, myd88, tube, pelle, traf6, imd, tak1, and relish) in the intestines (P < 0.05). In conclusion, dietary EMP supplementation enhances growth, promotes molting, and strengthens the innate immune response, demonstrating its potential as an alternative protein source and immune enhancer for mud crabs. |
| format | Article |
| id | doaj-art-81b6cf36b8a148fb85a89c9a441b9f11 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-81b6cf36b8a148fb85a89c9a441b9f112025-08-20T03:59:40ZengElsevierAquaculture Reports2352-51342025-09-014310300410.1016/j.aqrep.2025.103004Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain)Tiantian Xu0Xiaoyue Li1Min Jin2Zheng Tang3Xishuai Cui4Yinzhao Zhang5Feng Tang6Qicun Zhou7Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaZhejiang Fengyu Marine Organism Products Co., LTD., Zhoushan 316000, ChinaZhejiang Fengyu Marine Organism Products Co., LTD., Zhoushan 316000, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, China; Corresponding author at: Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.A feeding trial was conducted to evaluate the enzyme-hydrolyzed marine proteins (EMP, comprising fish protein peptides, enzymatic squid protein, and seaweed polysaccharides) supplementation on the growth performance, protein metabolism and immune responses of mud crabs (Scylla paramamosain). Four isonitrogenous and isolipidic diets were prepared with different treatments: a normal control (NC) containing 30 % FM, a positive control (PC) with 30 % FM supplemented with 2 % EMP, an experimental diet with 28 % FM and 1 % EMP (EMP1), and another experimental diet with 26 % FM and 2 % EMP (EMP2). A total of 120 healthy mud crabs were randomly assigned to individual crab apartments, with three replicates per treatment (10 crabs per replicate). Results showed that crabs given the EMP2 diet exhibited the highest PWG and SGR (P < 0.05). Crabs on the NC diet showed substantially decreased pepsin and amylase activities in the hepatopancreas and stomach compared to other diets (P < 0.05). Dietary EMP supplementation significantly upregulated the expression of molting-related genes (ecr, met, chi, e75, chh, and famet) in the hepatopancreas, protein metabolism-related genes (akt, rheb, tor, pi3k, irs, s6k, eif4e, 4ebp1, and 4ebp2) in both the hepatopancreas and intestines, and immune-related genes (lysozyme, proPO, toll, myd88, tube, pelle, traf6, imd, tak1, and relish) in the intestines (P < 0.05). In conclusion, dietary EMP supplementation enhances growth, promotes molting, and strengthens the innate immune response, demonstrating its potential as an alternative protein source and immune enhancer for mud crabs.http://www.sciencedirect.com/science/article/pii/S2352513425003904Mud crab (Scylla paramamosain)Enzyme-hydrolyzed marine proteinsGrowthProtein metabolismImmune response |
| spellingShingle | Tiantian Xu Xiaoyue Li Min Jin Zheng Tang Xishuai Cui Yinzhao Zhang Feng Tang Qicun Zhou Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) Aquaculture Reports Mud crab (Scylla paramamosain) Enzyme-hydrolyzed marine proteins Growth Protein metabolism Immune response |
| title | Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) |
| title_full | Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) |
| title_fullStr | Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) |
| title_full_unstemmed | Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) |
| title_short | Dietary enzyme-hydrolyzed marine proteins supplementation could improve growth, protein metabolism and immune response in mud crab (Scylla paramamosain) |
| title_sort | dietary enzyme hydrolyzed marine proteins supplementation could improve growth protein metabolism and immune response in mud crab scylla paramamosain |
| topic | Mud crab (Scylla paramamosain) Enzyme-hydrolyzed marine proteins Growth Protein metabolism Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003904 |
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