Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes
This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radi...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2021-0003 |
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| author | Zorić Nataša Kopjar Nevenka Rodriguez Jadranka Vuković Tomić Siniša Kosalec Ivan |
| author_facet | Zorić Nataša Kopjar Nevenka Rodriguez Jadranka Vuković Tomić Siniša Kosalec Ivan |
| author_sort | Zorić Nataša |
| collection | DOAJ |
| description | This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L−1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy. |
| format | Article |
| id | doaj-art-81223a4be88845fd9f9ed3aab4e9bb25 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-81223a4be88845fd9f9ed3aab4e9bb252025-02-02T09:52:47ZengSciendoActa Pharmaceutica1846-95582021-03-0171113114110.2478/acph-2021-0003acph-2021-0003Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytesZorić Nataša0Kopjar Nevenka1Rodriguez Jadranka Vuković2Tomić Siniša3Kosalec Ivan4Agency for Medicinal Products and Medical Devices of Croatia (HALMED)10000Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000ZagrebCroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry Department of Pharmaceutical Analysis10000Zagreb, CroatiaAgency for Medicinal Products and Medical Devices of Croatia (HALMED)10000Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry Department of Microbiology, 10000Zagreb, CroatiaThis study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L−1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy.https://doi.org/10.2478/acph-2021-0003oleuropeinhydroxytyrosololiveantioxidant activitydna damagehuman lymphocytescomet assay |
| spellingShingle | Zorić Nataša Kopjar Nevenka Rodriguez Jadranka Vuković Tomić Siniša Kosalec Ivan Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes Acta Pharmaceutica oleuropein hydroxytyrosol olive antioxidant activity dna damage human lymphocytes comet assay |
| title | Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes |
| title_full | Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes |
| title_fullStr | Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes |
| title_full_unstemmed | Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes |
| title_short | Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes |
| title_sort | protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide induced dna damage in human peripheral lymphocytes |
| topic | oleuropein hydroxytyrosol olive antioxidant activity dna damage human lymphocytes comet assay |
| url | https://doi.org/10.2478/acph-2021-0003 |
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