PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation
The low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and p...
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Elsevier
2025-02-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S193652332400384X |
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| author | Yaoxu Li Bing Li Kai Yang Lihua Zhu Hong Tang Yinpei Huang Jinhai Deng Jun Duan |
| author_facet | Yaoxu Li Bing Li Kai Yang Lihua Zhu Hong Tang Yinpei Huang Jinhai Deng Jun Duan |
| author_sort | Yaoxu Li |
| collection | DOAJ |
| description | The low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and promotes tumor metastasis. Here, we investigated the role and molecular mechanism of PER3 in regulating HIF-1α and metastasis in oral squamous cell carcinoma (OSCC) by using bioinformatics analyses and in vitro and in vivo experiments. PER3 expression was decreased in OSCC, and PER3 expression was significantly negatively correlated with T stage, N stage, clinical classification, and survival time. PER3 overexpression inhibited, while PER3 knockdown prompted EMT and metastasis of OSCC cells. HIF-1α reversed the effects of alterations in PER3 expression on OSCC metastasis. Mechanistically, PER3 bound to HIF-1α via the Per-ARNT-Sim 1 domain and promoted its ubiquitination degradation. Hypermethylation at CpG site cg12258811 of PER3 promoter inhibited PER3 expression and prognosis of OSCC. Decitabine combined with LW6 upregulated PER3, downregulated HIF-1α, and inhibited lymph node metastasis of OSCC in nude mice. Our findings reveal the role and mechanism of HIF-1α regulation by PER3 and support the potential clinical application of targeting PER3 in treating OSCC metastasis. |
| format | Article |
| id | doaj-art-810b8d4432a94f978d9e80bbe59fe5b2 |
| institution | Kabale University |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-810b8d4432a94f978d9e80bbe59fe5b22025-01-22T05:41:30ZengElsevierTranslational Oncology1936-52332025-02-0152102258PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradationYaoxu Li0Bing Li1Kai Yang2Lihua Zhu3Hong Tang4Yinpei Huang5Jinhai Deng6Jun Duan7Department of Stomatology, Chongqing University Three Gorges Hospital, School of Medicine, Chongqing University, Wanzhou District, Chongqing, 404100, PR China; Clinical Research Center (CRC), Medical Pathology Center (MPC), Cancer Early Detection and Treatment Center (CEDTC) and Translational Medicine Research Center (TMRC), Chongqing University Three Gorges Hospital, School of Medicine, Chongqing University, Wanzhou District, Chongqing, 404100, PR China; Corresponding authors.Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China; Corresponding authors.Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China; Corresponding authors.Department of General Practice, Chongqing University Three Gorges Hospital, School of Medicine, Chongqing University, Wanzhou District, Chongqing, 404100, PR ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaDepartment of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaClinical Research Center (CRC), Medical Pathology Center (MPC), Cancer Early Detection and Treatment Center (CEDTC) and Translational Medicine Research Center (TMRC), Chongqing University Three Gorges Hospital, School of Medicine, Chongqing University, Wanzhou District, Chongqing, 404100, PR ChinaDepartment of Stomatology, Children's Hospital of Chongqing Medical University, Chongqing, PR ChinaThe low expression of period circadian regulator 3 (PER3) in head and neck squamous cell carcinoma is closely correlated with tumor size and invasion depth. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) regulates epithelial-mesenchymal transition (EMT) transcription factors, activates EMT, and promotes tumor metastasis. Here, we investigated the role and molecular mechanism of PER3 in regulating HIF-1α and metastasis in oral squamous cell carcinoma (OSCC) by using bioinformatics analyses and in vitro and in vivo experiments. PER3 expression was decreased in OSCC, and PER3 expression was significantly negatively correlated with T stage, N stage, clinical classification, and survival time. PER3 overexpression inhibited, while PER3 knockdown prompted EMT and metastasis of OSCC cells. HIF-1α reversed the effects of alterations in PER3 expression on OSCC metastasis. Mechanistically, PER3 bound to HIF-1α via the Per-ARNT-Sim 1 domain and promoted its ubiquitination degradation. Hypermethylation at CpG site cg12258811 of PER3 promoter inhibited PER3 expression and prognosis of OSCC. Decitabine combined with LW6 upregulated PER3, downregulated HIF-1α, and inhibited lymph node metastasis of OSCC in nude mice. Our findings reveal the role and mechanism of HIF-1α regulation by PER3 and support the potential clinical application of targeting PER3 in treating OSCC metastasis.http://www.sciencedirect.com/science/article/pii/S193652332400384XOral squamous cell carcinomaMetastasisHypoxia-inducible factor 1 subunit alphaPeriod circadian regulator 3Promoter methylation |
| spellingShingle | Yaoxu Li Bing Li Kai Yang Lihua Zhu Hong Tang Yinpei Huang Jinhai Deng Jun Duan PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation Translational Oncology Oral squamous cell carcinoma Metastasis Hypoxia-inducible factor 1 subunit alpha Period circadian regulator 3 Promoter methylation |
| title | PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation |
| title_full | PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation |
| title_fullStr | PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation |
| title_full_unstemmed | PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation |
| title_short | PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation |
| title_sort | per3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting hif 1α degradation |
| topic | Oral squamous cell carcinoma Metastasis Hypoxia-inducible factor 1 subunit alpha Period circadian regulator 3 Promoter methylation |
| url | http://www.sciencedirect.com/science/article/pii/S193652332400384X |
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