Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.)
Abstract Riccia sorocarpa Bisch., commonly known as common crystalwort, is a plant belonging to the Marchantiales order with a cosmopolitan distribution among a wide range of habitats: fields, gardens, waste ground, on paths, cliff tops, and thin soil over rocks or by water bodies. However, research...
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Nature Portfolio
2025-01-01
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| author | Katarzyna Krawczyk Joanna Szablińska-Piernik Łukasz Paukszto Mateusz Maździarz Paweł Sulima Jerzy Andrzej Przyborowski Monika Szczecińska Jakub Sawicki |
| author_facet | Katarzyna Krawczyk Joanna Szablińska-Piernik Łukasz Paukszto Mateusz Maździarz Paweł Sulima Jerzy Andrzej Przyborowski Monika Szczecińska Jakub Sawicki |
| author_sort | Katarzyna Krawczyk |
| collection | DOAJ |
| description | Abstract Riccia sorocarpa Bisch., commonly known as common crystalwort, is a plant belonging to the Marchantiales order with a cosmopolitan distribution among a wide range of habitats: fields, gardens, waste ground, on paths, cliff tops, and thin soil over rocks or by water bodies. However, research into the genetic aspects of this species is limited. In this study, the chromosome-scale telomere-to-telomere genome of R. sorocarpa was assembled exclusively by Oxford Nanopore long-read sequencing and Pore-C technology. A high-quality chromosomal-scale assembly was obtained with a final genome size of 376.690 Mbp, contig N50 of 49.132 Mbp and 97.02% of the assembled contigs associated with the eight chromosomes. Genome assembly completeness was confirmed by BUSCO analysis accounting 91.8%. Among 27,626 total genes, 23,562 (85.29%) were functionally annotated. Moreover, collinearity of Marchantiales was analyzed as well as gene family evolution and DNA methylation profile. The availability of this genome, which is the second telomere-to-telomere liverwort assembly, opens up new avenues for in-depth analysis of R. sorocarpa genetic diversity and genomic characteristics. |
| format | Article |
| id | doaj-art-8102c1d79f9143e6bf97a25545413f7e |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-8102c1d79f9143e6bf97a25545413f7e2025-01-19T12:10:06ZengNature PortfolioScientific Data2052-44632025-01-0112111110.1038/s41597-025-04373-6Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.)Katarzyna Krawczyk0Joanna Szablińska-Piernik1Łukasz Paukszto2Mateusz Maździarz3Paweł Sulima4Jerzy Andrzej Przyborowski5Monika Szczecińska6Jakub Sawicki7Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Genetics, Plant Breeding and Bioresource Engineering, University of Warmia and Mazury in OlsztynDepartment of Genetics, Plant Breeding and Bioresource Engineering, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynAbstract Riccia sorocarpa Bisch., commonly known as common crystalwort, is a plant belonging to the Marchantiales order with a cosmopolitan distribution among a wide range of habitats: fields, gardens, waste ground, on paths, cliff tops, and thin soil over rocks or by water bodies. However, research into the genetic aspects of this species is limited. In this study, the chromosome-scale telomere-to-telomere genome of R. sorocarpa was assembled exclusively by Oxford Nanopore long-read sequencing and Pore-C technology. A high-quality chromosomal-scale assembly was obtained with a final genome size of 376.690 Mbp, contig N50 of 49.132 Mbp and 97.02% of the assembled contigs associated with the eight chromosomes. Genome assembly completeness was confirmed by BUSCO analysis accounting 91.8%. Among 27,626 total genes, 23,562 (85.29%) were functionally annotated. Moreover, collinearity of Marchantiales was analyzed as well as gene family evolution and DNA methylation profile. The availability of this genome, which is the second telomere-to-telomere liverwort assembly, opens up new avenues for in-depth analysis of R. sorocarpa genetic diversity and genomic characteristics.https://doi.org/10.1038/s41597-025-04373-6 |
| spellingShingle | Katarzyna Krawczyk Joanna Szablińska-Piernik Łukasz Paukszto Mateusz Maździarz Paweł Sulima Jerzy Andrzej Przyborowski Monika Szczecińska Jakub Sawicki Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) Scientific Data |
| title | Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) |
| title_full | Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) |
| title_fullStr | Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) |
| title_full_unstemmed | Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) |
| title_short | Chromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.) |
| title_sort | chromosome scale telomere to telomere genome assembly of common crystalwort riccia sorocarpa bisch |
| url | https://doi.org/10.1038/s41597-025-04373-6 |
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