Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights
Platelet cells are essential to maintain haemostasis and play a critical role in thrombosis. They swiftly respond to vascular injury by adhering to damaged vessel surfaces, activating signalling pathways, and aggregating with each other to control bleeding. This dynamic process of platelet activatio...
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MDPI AG
2025-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/491 |
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| author | Yuping Yolanda Tan Jinghan Liu Qian Peter Su |
| author_facet | Yuping Yolanda Tan Jinghan Liu Qian Peter Su |
| author_sort | Yuping Yolanda Tan |
| collection | DOAJ |
| description | Platelet cells are essential to maintain haemostasis and play a critical role in thrombosis. They swiftly respond to vascular injury by adhering to damaged vessel surfaces, activating signalling pathways, and aggregating with each other to control bleeding. This dynamic process of platelet activation is intricately coordinated, spanning from membrane receptor maturation to intracellular interactions to whole-cell responses. Live-cell imaging has become an invaluable tool for dissecting these complexes. Despite its benefits, live imaging of platelets presents significant technical challenges. This review addresses these challenges, identifying key areas in need of further development and proposing possible solutions. We also focus on the dynamic processes of platelet adhesion, activation, and aggregation in haemostasis and thrombosis, applying imaging capacities from the microscale to the nanoscale. By exploring various live imaging techniques, we demonstrate how these approaches offer crucial insights into platelet biology and deepen our understanding of these three core events. In conclusion, this review provides an overview of the imaging methods currently available for studying platelet dynamics, guiding researchers in selecting suitable techniques for specific studies. By advancing our knowledge of platelet behaviour, these imaging methods contribute to research on haemostasis, thrombosis, and platelet-related diseases, ultimately aiming to improve clinical outcomes. |
| format | Article |
| id | doaj-art-80eb5d6d23464a6da02a0e7a6c0ce42c |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-80eb5d6d23464a6da02a0e7a6c0ce42c2025-01-24T13:49:07ZengMDPI AGSensors1424-82202025-01-0125249110.3390/s25020491Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and InsightsYuping Yolanda Tan0Jinghan Liu1Qian Peter Su2School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaPlatelet cells are essential to maintain haemostasis and play a critical role in thrombosis. They swiftly respond to vascular injury by adhering to damaged vessel surfaces, activating signalling pathways, and aggregating with each other to control bleeding. This dynamic process of platelet activation is intricately coordinated, spanning from membrane receptor maturation to intracellular interactions to whole-cell responses. Live-cell imaging has become an invaluable tool for dissecting these complexes. Despite its benefits, live imaging of platelets presents significant technical challenges. This review addresses these challenges, identifying key areas in need of further development and proposing possible solutions. We also focus on the dynamic processes of platelet adhesion, activation, and aggregation in haemostasis and thrombosis, applying imaging capacities from the microscale to the nanoscale. By exploring various live imaging techniques, we demonstrate how these approaches offer crucial insights into platelet biology and deepen our understanding of these three core events. In conclusion, this review provides an overview of the imaging methods currently available for studying platelet dynamics, guiding researchers in selecting suitable techniques for specific studies. By advancing our knowledge of platelet behaviour, these imaging methods contribute to research on haemostasis, thrombosis, and platelet-related diseases, ultimately aiming to improve clinical outcomes.https://www.mdpi.com/1424-8220/25/2/491plateletsplatelet adhesionplatelet activationplatelet aggregationplatelet diseaseslive-cell imaging |
| spellingShingle | Yuping Yolanda Tan Jinghan Liu Qian Peter Su Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights Sensors platelets platelet adhesion platelet activation platelet aggregation platelet diseases live-cell imaging |
| title | Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights |
| title_full | Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights |
| title_fullStr | Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights |
| title_full_unstemmed | Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights |
| title_short | Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights |
| title_sort | advancing platelet research through live cell imaging challenges techniques and insights |
| topic | platelets platelet adhesion platelet activation platelet aggregation platelet diseases live-cell imaging |
| url | https://www.mdpi.com/1424-8220/25/2/491 |
| work_keys_str_mv | AT yupingyolandatan advancingplateletresearchthroughlivecellimagingchallengestechniquesandinsights AT jinghanliu advancingplateletresearchthroughlivecellimagingchallengestechniquesandinsights AT qianpetersu advancingplateletresearchthroughlivecellimagingchallengestechniquesandinsights |