Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands
Abstract Current estimates of wetland contributions to the global methane budget carry high uncertainty, particularly in accurately predicting emissions from high methane-emitting wetlands. Microorganisms drive methane cycling, but little is known about their conservation across wetlands. To address...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56133-0 |
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| author | Emily K. Bechtold Jared B. Ellenbogen Jorge A. Villa Djennyfer K. de Melo Ferreira Angela M. Oliverio Joel E. Kostka Virginia I. Rich Ruth K. Varner Sheel Bansal Eric J. Ward Gil Bohrer Mikayla A. Borton Kelly C. Wrighton Michael J. Wilkins |
| author_facet | Emily K. Bechtold Jared B. Ellenbogen Jorge A. Villa Djennyfer K. de Melo Ferreira Angela M. Oliverio Joel E. Kostka Virginia I. Rich Ruth K. Varner Sheel Bansal Eric J. Ward Gil Bohrer Mikayla A. Borton Kelly C. Wrighton Michael J. Wilkins |
| author_sort | Emily K. Bechtold |
| collection | DOAJ |
| description | Abstract Current estimates of wetland contributions to the global methane budget carry high uncertainty, particularly in accurately predicting emissions from high methane-emitting wetlands. Microorganisms drive methane cycling, but little is known about their conservation across wetlands. To address this, we integrate 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux data across 9 wetlands, creating the Multi-Omics for Understanding Climate Change (MUCC) v2.0.0 database. This resource is used to link microbiome composition to function and methane emissions, focusing on methane-cycling microbes and the networks driving carbon decomposition. We identify eight methane-cycling genera shared across wetlands and show wetland-specific metabolic interactions in marshes, revealing low connections between methanogens and methanotrophs in high-emitting wetlands. Methanoregula emerged as a hub methanogen across networks and is a strong predictor of methane flux. In these wetlands it also displays the functional potential for methylotrophic methanogenesis, highlighting the importance of this pathway in these ecosystems. Collectively, our findings illuminate trends between microbial decomposition networks and methane flux while providing an extensive publicly available database to advance future wetland research. |
| format | Article |
| id | doaj-art-80e6f277d8f14d69b9be53ec5c77be32 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-80e6f277d8f14d69b9be53ec5c77be322025-01-26T12:41:16ZengNature PortfolioNature Communications2041-17232025-01-0116111510.1038/s41467-025-56133-0Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlandsEmily K. Bechtold0Jared B. Ellenbogen1Jorge A. Villa2Djennyfer K. de Melo Ferreira3Angela M. Oliverio4Joel E. Kostka5Virginia I. Rich6Ruth K. Varner7Sheel Bansal8Eric J. Ward9Gil Bohrer10Mikayla A. Borton11Kelly C. Wrighton12Michael J. Wilkins13Department of Soil and Crop Sciences, Colorado State UniversityDepartment of Soil and Crop Sciences, Colorado State UniversitySchool of Geosciences, University of Louisiana at LafayetteDepartment of Soil and Crop Sciences, Colorado State UniversityDepartment of Soil and Crop Sciences, Colorado State UniversitySchool of Biological Sciences, Georgia Institute of TechnologyDepartment of Microbiology, The Ohio State UniversityDepartment of Earth Sciences and Institute for the Study of Earth, Oceans and Space, University of New HampshireUnited States Geological SurveyUniversity of MarylandDepartment of Civil, Environmental & Geodetic Engineering, The Ohio State UniversityDepartment of Soil and Crop Sciences, Colorado State UniversityDepartment of Soil and Crop Sciences, Colorado State UniversityDepartment of Soil and Crop Sciences, Colorado State UniversityAbstract Current estimates of wetland contributions to the global methane budget carry high uncertainty, particularly in accurately predicting emissions from high methane-emitting wetlands. Microorganisms drive methane cycling, but little is known about their conservation across wetlands. To address this, we integrate 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux data across 9 wetlands, creating the Multi-Omics for Understanding Climate Change (MUCC) v2.0.0 database. This resource is used to link microbiome composition to function and methane emissions, focusing on methane-cycling microbes and the networks driving carbon decomposition. We identify eight methane-cycling genera shared across wetlands and show wetland-specific metabolic interactions in marshes, revealing low connections between methanogens and methanotrophs in high-emitting wetlands. Methanoregula emerged as a hub methanogen across networks and is a strong predictor of methane flux. In these wetlands it also displays the functional potential for methylotrophic methanogenesis, highlighting the importance of this pathway in these ecosystems. Collectively, our findings illuminate trends between microbial decomposition networks and methane flux while providing an extensive publicly available database to advance future wetland research.https://doi.org/10.1038/s41467-025-56133-0 |
| spellingShingle | Emily K. Bechtold Jared B. Ellenbogen Jorge A. Villa Djennyfer K. de Melo Ferreira Angela M. Oliverio Joel E. Kostka Virginia I. Rich Ruth K. Varner Sheel Bansal Eric J. Ward Gil Bohrer Mikayla A. Borton Kelly C. Wrighton Michael J. Wilkins Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands Nature Communications |
| title | Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| title_full | Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| title_fullStr | Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| title_full_unstemmed | Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| title_short | Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| title_sort | metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands |
| url | https://doi.org/10.1038/s41467-025-56133-0 |
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