Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice
Abstract The serotonergic system modulates the neural circuits involved in jaw movement; however, the role of serotonin (5-HT) neurons in masticatory movement remains unclear. Here, we investigated the effect of selective activation of 5-HT neurons in the dorsal raphe nucleus (DRN), or the raphe obs...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-11-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-79429-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850184679617986560 |
|---|---|
| author | Masanori Dantsuji Ayako Mochizuki Kiyomi Nakayama Mitsuko Kanamaru Masahiko Izumizaki Kenji F. Tanaka Tomio Inoue Shiro Nakamura |
| author_facet | Masanori Dantsuji Ayako Mochizuki Kiyomi Nakayama Mitsuko Kanamaru Masahiko Izumizaki Kenji F. Tanaka Tomio Inoue Shiro Nakamura |
| author_sort | Masanori Dantsuji |
| collection | DOAJ |
| description | Abstract The serotonergic system modulates the neural circuits involved in jaw movement; however, the role of serotonin (5-HT) neurons in masticatory movement remains unclear. Here, we investigated the effect of selective activation of 5-HT neurons in the dorsal raphe nucleus (DRN), or the raphe obscurus nucleus (ROb), on voluntary masticatory movement using transgenic mice expressing the channelrhodopsin-2 (ChR2) mutant (C128S) in central 5-HT neurons. During voluntary mastication, DRN blue light illumination increased masticatory frequency and decreased the root mean square peak amplitude of electromyography (EMG) in the masseter muscles. DRN blue light illumination also decreased EMG burst duration in the masseter and digastric muscles. These changes were blocked by a 5-HT2A receptor antagonist. Conversely, ROb blue light illumination during voluntary mastication did not affect masticatory frequency and EMG bursts in the masseter and digastric muscles. DRN or ROb blue light illumination during the resting state did not induce rhythmic jaw movement such as mastication but induced an increase in EMG activity in masseter and digastric muscles. These results suggest that both DRN and ROb 5-HT neurons may facilitate jaw movement. Furthermore, DRN 5-HT neuron may contribute to changes in masticatory patterns during the masticatory sequence. |
| format | Article |
| id | doaj-art-80e1bd2e36e94e95a63fb8a8b7005d58 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-80e1bd2e36e94e95a63fb8a8b7005d582025-08-20T02:16:59ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-79429-5Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving miceMasanori Dantsuji0Ayako Mochizuki1Kiyomi Nakayama2Mitsuko Kanamaru3Masahiko Izumizaki4Kenji F. Tanaka5Tomio Inoue6Shiro Nakamura7Department of Oral Physiology, Showa University School of DentistryDepartment of Oral Physiology, Showa University School of DentistryDepartment of Oral Physiology, Showa University School of DentistryFaculty of Arts and Sciences at Fujiyoshida, Showa UniversityDepartment of Physiology, Showa University School of MedicineDivision of Brain Sciences, Institute for Advanced Medical Research, Keio University School of MedicineDepartment of Oral Physiology, Showa University School of DentistryDepartment of Oral Physiology, Showa University School of DentistryAbstract The serotonergic system modulates the neural circuits involved in jaw movement; however, the role of serotonin (5-HT) neurons in masticatory movement remains unclear. Here, we investigated the effect of selective activation of 5-HT neurons in the dorsal raphe nucleus (DRN), or the raphe obscurus nucleus (ROb), on voluntary masticatory movement using transgenic mice expressing the channelrhodopsin-2 (ChR2) mutant (C128S) in central 5-HT neurons. During voluntary mastication, DRN blue light illumination increased masticatory frequency and decreased the root mean square peak amplitude of electromyography (EMG) in the masseter muscles. DRN blue light illumination also decreased EMG burst duration in the masseter and digastric muscles. These changes were blocked by a 5-HT2A receptor antagonist. Conversely, ROb blue light illumination during voluntary mastication did not affect masticatory frequency and EMG bursts in the masseter and digastric muscles. DRN or ROb blue light illumination during the resting state did not induce rhythmic jaw movement such as mastication but induced an increase in EMG activity in masseter and digastric muscles. These results suggest that both DRN and ROb 5-HT neurons may facilitate jaw movement. Furthermore, DRN 5-HT neuron may contribute to changes in masticatory patterns during the masticatory sequence.https://doi.org/10.1038/s41598-024-79429-5MasticationSerotoninDorsal rapheChannelrhodopsinsOptogeneticsElectromyography |
| spellingShingle | Masanori Dantsuji Ayako Mochizuki Kiyomi Nakayama Mitsuko Kanamaru Masahiko Izumizaki Kenji F. Tanaka Tomio Inoue Shiro Nakamura Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice Scientific Reports Mastication Serotonin Dorsal raphe Channelrhodopsins Optogenetics Electromyography |
| title | Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| title_full | Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| title_fullStr | Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| title_full_unstemmed | Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| title_short | Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| title_sort | optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice |
| topic | Mastication Serotonin Dorsal raphe Channelrhodopsins Optogenetics Electromyography |
| url | https://doi.org/10.1038/s41598-024-79429-5 |
| work_keys_str_mv | AT masanoridantsuji optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT ayakomochizuki optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT kiyominakayama optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT mitsukokanamaru optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT masahikoizumizaki optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT kenjiftanaka optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT tomioinoue optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice AT shironakamura optogeneticactivationofserotonergicneuronschangesmasticatorymovementinfreelymovingmice |