Elevated expression of galectin-3 in excessive scars: A pilot study
Introduction: Hypertrophic scars and keloids are types of excessive scars from abnormal skin healing. Galectin-3 (gal-3) is a protein from the lectin family capable of identifying carbohydrates, which can combine and act on different molecules. In the literature, the action of gal-3 as the main regu...
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| Format: | Article |
| Language: | English |
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Thieme Revinter Publicações Ltda.
2024-10-01
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| Series: | Revista Brasileira de Cirurgia Plástica |
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| Online Access: | http://www.rbcp.org.br/export-pdf/3482/1983-5175-rbcp-39-03-e936-en.pdf |
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| author | Victor Freire Salomão Ferreira Felipe Simões Lemos Roberto Stefan de Almeida Ribeiro Silvio Renan Pinheiro Victor de-Araújo Fabio Santiago Figueredo Maria Lidia de Abreu Silva Felipe Leite de-Oliveira Thaís Porto Amadeu |
| author_facet | Victor Freire Salomão Ferreira Felipe Simões Lemos Roberto Stefan de Almeida Ribeiro Silvio Renan Pinheiro Victor de-Araújo Fabio Santiago Figueredo Maria Lidia de Abreu Silva Felipe Leite de-Oliveira Thaís Porto Amadeu |
| author_sort | Victor Freire Salomão Ferreira |
| collection | DOAJ |
| description | Introduction: Hypertrophic scars and keloids are types of excessive scars from abnormal skin healing. Galectin-3 (gal-3) is a protein from the lectin family capable of identifying carbohydrates, which can combine and act on different molecules. In the literature, the action of gal-3 as the main regulatory agent of fibrogenesis has already been described and is currently used in anti-fibrotic therapy for various organs such as the lung and liver. The objective of this pilot study was to show preliminary results found in the expression of gal-3 in exacerbated scars.
Method: Twenty biopsy samples from excessive scars (16 keloids and 4 hypertrophic scars) and 9 samples from normal scars were collected from 22 women and 7 men. These samples were processed for routine histopathological analysis by immunohistochemistry to detect gal-3. Gal-3 positive cells were quantified by the stereological method using a 36-point grid.
Results: Immunohistochemistry showed high expression of gal-3 in endothelial and epithelial cells of all scar samples, as well as expression in cells distributed throughout the dermis. Higher gal-3 expression was found in keloid samples (28% positive cells) compared to normal (18%) and hypertrophic (22%) scars (p = 0.0075). The results were obtained from a small number of patients, as this was a pilot study.
Conclusion: The data suggest that gal-3 participates in the healing process and, due to its greater presence in keloid samples, it may be a potential biomarker for keloid formation and a promising therapeutic target to be explored. |
| format | Article |
| id | doaj-art-80c4b60552e64732abe4d43f4f46b6b1 |
| institution | Kabale University |
| issn | 1983-5175 2177-1235 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | Revista Brasileira de Cirurgia Plástica |
| spelling | doaj-art-80c4b60552e64732abe4d43f4f46b6b12025-08-20T03:57:03ZengThieme Revinter Publicações Ltda.Revista Brasileira de Cirurgia Plástica1983-51752177-12352024-10-0139310.5935/2177-1235.2024RBCP0936-ENElevated expression of galectin-3 in excessive scars: A pilot studyVictor Freire Salomão Ferreira0Felipe Simões Lemos1Roberto Stefan de Almeida Ribeiro2Silvio Renan Pinheiro Victor de-Araújo3Fabio Santiago Figueredo4Maria Lidia de Abreu Silva5Felipe Leite de-Oliveira6Thaís Porto Amadeu7Universidade do Estado do Rio de Janeiro, Patologia Geral, Laboratório de Imunopatologia, Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro, Patologia Geral, Laboratório de Imunopatologia, Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro, Patologia Geral, Laboratório de Imunopatologia, Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro, Patologia Geral, Laboratório de Imunopatologia, Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro, Setor de Cirurgia Plástica, Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Rio de Janeiro, RJ, Brazil.Universidade do Estado do Rio de Janeiro, Patologia Geral, Laboratório de Imunopatologia, Rio de Janeiro, RJ, Brazil.Introduction: Hypertrophic scars and keloids are types of excessive scars from abnormal skin healing. Galectin-3 (gal-3) is a protein from the lectin family capable of identifying carbohydrates, which can combine and act on different molecules. In the literature, the action of gal-3 as the main regulatory agent of fibrogenesis has already been described and is currently used in anti-fibrotic therapy for various organs such as the lung and liver. The objective of this pilot study was to show preliminary results found in the expression of gal-3 in exacerbated scars. Method: Twenty biopsy samples from excessive scars (16 keloids and 4 hypertrophic scars) and 9 samples from normal scars were collected from 22 women and 7 men. These samples were processed for routine histopathological analysis by immunohistochemistry to detect gal-3. Gal-3 positive cells were quantified by the stereological method using a 36-point grid. Results: Immunohistochemistry showed high expression of gal-3 in endothelial and epithelial cells of all scar samples, as well as expression in cells distributed throughout the dermis. Higher gal-3 expression was found in keloid samples (28% positive cells) compared to normal (18%) and hypertrophic (22%) scars (p = 0.0075). The results were obtained from a small number of patients, as this was a pilot study. Conclusion: The data suggest that gal-3 participates in the healing process and, due to its greater presence in keloid samples, it may be a potential biomarker for keloid formation and a promising therapeutic target to be explored.http://www.rbcp.org.br/export-pdf/3482/1983-5175-rbcp-39-03-e936-en.pdfimmunohistochemistrykeloidcicatrix, hypertrophicgalectin 3wound healing |
| spellingShingle | Victor Freire Salomão Ferreira Felipe Simões Lemos Roberto Stefan de Almeida Ribeiro Silvio Renan Pinheiro Victor de-Araújo Fabio Santiago Figueredo Maria Lidia de Abreu Silva Felipe Leite de-Oliveira Thaís Porto Amadeu Elevated expression of galectin-3 in excessive scars: A pilot study Revista Brasileira de Cirurgia Plástica immunohistochemistry keloid cicatrix, hypertrophic galectin 3 wound healing |
| title | Elevated expression of galectin-3 in excessive scars: A pilot study |
| title_full | Elevated expression of galectin-3 in excessive scars: A pilot study |
| title_fullStr | Elevated expression of galectin-3 in excessive scars: A pilot study |
| title_full_unstemmed | Elevated expression of galectin-3 in excessive scars: A pilot study |
| title_short | Elevated expression of galectin-3 in excessive scars: A pilot study |
| title_sort | elevated expression of galectin 3 in excessive scars a pilot study |
| topic | immunohistochemistry keloid cicatrix, hypertrophic galectin 3 wound healing |
| url | http://www.rbcp.org.br/export-pdf/3482/1983-5175-rbcp-39-03-e936-en.pdf |
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