Purification and Identification of an Antimicrobial Protein from <i>Bacillus stercoris</i> TY-12 and Its Biocontrol Functions Against <i>Ralstonia solanacearum</i>
<i>Ralstonia solanacearum</i> is a bacterial pathogen that causes bacterial wilt in plants, resulting in significant economic losses worldwide. Biological control that mainly utilizes <i>Bacillus</i> spp. is one of the most effective methods to prevent this disease. In this w...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Applied Microbiology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8007/5/1/2 |
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| Summary: | <i>Ralstonia solanacearum</i> is a bacterial pathogen that causes bacterial wilt in plants, resulting in significant economic losses worldwide. Biological control that mainly utilizes <i>Bacillus</i> spp. is one of the most effective methods to prevent this disease. In this work, a strain of <i>Bacillus stercoris</i> TY-12 with an obvious antagonism effect on <i>R. solanacearum</i> was screened, and the inhibition diameter against <i>R. solanacearum</i> reached 2.18 cm by the plate antagonism test. Furthermore, an antimicrobial protein was isolated and purified from the fermentation supernatant of TY-12. The LC-MS/MS analysis results indicated that the purified antimicrobial protein is a member of the M42 family metallopeptidase with a molecular weight of approximately 40 kDa and named MP-TY12. After co-culture with MP-TY12 for 4 h, the cell surface of <i>R. solanacearum</i> was disrupted under SEM, indicating that MP-TY12 may inhibit <i>R. solanacearum</i> growth by enzymatically cleaving peptide bonds within the cell wall or membrane structure via hydrolysis. To evaluate the potential application of TY-12 in disease control during crop production, the biocontrol efficacy of TY-12 on the capsicum infected by <i>R. solanacearum</i> was investigated and achieved 84.18%. The growth promotion tests showed that the dry weight, fresh weight, stem diameter, stem length, root length, and the chlorophyll content of capsicum using TY-12 was obviously increased compared to the blank control. It is suggested that TY-12 could be used as a new biocontrol microbial strain in crop production and MP-TY12 might be developed as an antimicrobial agent. |
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| ISSN: | 2673-8007 |