in vitro activation of complement and contact system by lactic acidosis
The activation of complement and contact systems occurs in reperfusion injuries with initial tissue hypoxia, and lactic acidosis such as mycardial infarction and birth asphyxia. The aim of our experiment was the formal proof of activation by sole lactic acidosis. Lactic acid was added to blood and p...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359891388 |
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| author | J. Sonntag M. Emeis E. Strauss M. Obladen |
| author_facet | J. Sonntag M. Emeis E. Strauss M. Obladen |
| author_sort | J. Sonntag |
| collection | DOAJ |
| description | The activation of complement and contact systems occurs in reperfusion injuries with initial tissue hypoxia, and lactic acidosis such as mycardial infarction and birth asphyxia. The aim of our experiment was the formal proof of activation by sole lactic acidosis. Lactic acid was added to blood and plasma samples from 10 healthy volunteers. C5a and factor XIIa were measured by EIA after incubation at 37°C for 1 h. Both concentrations increased (P<0.0001 by Friedman analysis) in blood and plasma samples with increasing amount of added lactic acid. Lactic acidosis can activate C5 from the complement system and factor XII from the contact system directly, even in the absence of cellular components. |
| format | Article |
| id | doaj-art-80bcd3a7ff9f4f518b93ced5c5c56703 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-80bcd3a7ff9f4f518b93ced5c5c567032025-02-03T01:26:45ZengWileyMediators of Inflammation0962-93511466-18611998-01-0171495110.1080/09629359891388in vitro activation of complement and contact system by lactic acidosisJ. Sonntag0M. Emeis1E. Strauss2M. Obladen3Department of Neonatology, Charité-Virchow-Klinikum, Humboldt Universität Berlin, Berlin, GermanyDepartment of Neonatology, Charité-Virchow-Klinikum, Humboldt Universität Berlin, Berlin, GermanyDepartment of Neonatology, Charité-Virchow-Klinikum, Humboldt Universität Berlin, Berlin, GermanyDepartment of Neonatology, Charité-Virchow-Klinikum, Humboldt Universität Berlin, Berlin, GermanyThe activation of complement and contact systems occurs in reperfusion injuries with initial tissue hypoxia, and lactic acidosis such as mycardial infarction and birth asphyxia. The aim of our experiment was the formal proof of activation by sole lactic acidosis. Lactic acid was added to blood and plasma samples from 10 healthy volunteers. C5a and factor XIIa were measured by EIA after incubation at 37°C for 1 h. Both concentrations increased (P<0.0001 by Friedman analysis) in blood and plasma samples with increasing amount of added lactic acid. Lactic acidosis can activate C5 from the complement system and factor XII from the contact system directly, even in the absence of cellular components.http://dx.doi.org/10.1080/09629359891388Lactic acidosisComplement systemContact systemHageman factorC5a. |
| spellingShingle | J. Sonntag M. Emeis E. Strauss M. Obladen in vitro activation of complement and contact system by lactic acidosis Mediators of Inflammation Lactic acidosis Complement system Contact system Hageman factor C5a. |
| title | in vitro activation of complement and contact system by lactic acidosis |
| title_full | in vitro activation of complement and contact system by lactic acidosis |
| title_fullStr | in vitro activation of complement and contact system by lactic acidosis |
| title_full_unstemmed | in vitro activation of complement and contact system by lactic acidosis |
| title_short | in vitro activation of complement and contact system by lactic acidosis |
| title_sort | in vitro activation of complement and contact system by lactic acidosis |
| topic | Lactic acidosis Complement system Contact system Hageman factor C5a. |
| url | http://dx.doi.org/10.1080/09629359891388 |
| work_keys_str_mv | AT jsonntag invitroactivationofcomplementandcontactsystembylacticacidosis AT memeis invitroactivationofcomplementandcontactsystembylacticacidosis AT estrauss invitroactivationofcomplementandcontactsystembylacticacidosis AT mobladen invitroactivationofcomplementandcontactsystembylacticacidosis |