Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells
Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokine...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/183760 |
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| _version_ | 1832563029604040704 |
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| author | Shinichiro Kina Toshiyuki Nakasone Hiroyuki Takemoto Akira Matayoshi Shoko Makishi Nao Sunagawa Feixin Liang Thongsavanh Phonaphonh Hajime Sunakawa |
| author_facet | Shinichiro Kina Toshiyuki Nakasone Hiroyuki Takemoto Akira Matayoshi Shoko Makishi Nao Sunagawa Feixin Liang Thongsavanh Phonaphonh Hajime Sunakawa |
| author_sort | Shinichiro Kina |
| collection | DOAJ |
| description | Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used 𝑜-phenanthroline to deplete Fe2+ in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system.
The iron chelator 𝑜-phenanthroline effectively inhibited H2O2-induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When Fe2+ was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that Fe2+-mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells. |
| format | Article |
| id | doaj-art-80b0566a6ada450a97ccc556674bb338 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-80b0566a6ada450a97ccc556674bb3382025-02-03T01:21:10ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/183760183760Regulation of Chemokine Production via Oxidative Pathway in HeLa CellsShinichiro Kina0Toshiyuki Nakasone1Hiroyuki Takemoto2Akira Matayoshi3Shoko Makishi4Nao Sunagawa5Feixin Liang6Thongsavanh Phonaphonh7Hajime Sunakawa8Department of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanInflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used 𝑜-phenanthroline to deplete Fe2+ in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system. The iron chelator 𝑜-phenanthroline effectively inhibited H2O2-induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When Fe2+ was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that Fe2+-mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells.http://dx.doi.org/10.1155/2009/183760 |
| spellingShingle | Shinichiro Kina Toshiyuki Nakasone Hiroyuki Takemoto Akira Matayoshi Shoko Makishi Nao Sunagawa Feixin Liang Thongsavanh Phonaphonh Hajime Sunakawa Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells Mediators of Inflammation |
| title | Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells |
| title_full | Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells |
| title_fullStr | Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells |
| title_full_unstemmed | Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells |
| title_short | Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells |
| title_sort | regulation of chemokine production via oxidative pathway in hela cells |
| url | http://dx.doi.org/10.1155/2009/183760 |
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