The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis
Abstract Currently, the identification of valuable candidate genes affecting milk fat synthesis in dairy cows is still limited, and the specific regulatory mechanism is still unknown. In this study, we used primary bovine mammary epithelial cells(BMECs)as a model and utilized overexpression and knoc...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87089-2 |
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| author | Tong Mu Honghong Hu Xiaofang Feng Chuanchuan Wang Juan Zhang Zhihong Sun Yaling Gu |
| author_facet | Tong Mu Honghong Hu Xiaofang Feng Chuanchuan Wang Juan Zhang Zhihong Sun Yaling Gu |
| author_sort | Tong Mu |
| collection | DOAJ |
| description | Abstract Currently, the identification of valuable candidate genes affecting milk fat synthesis in dairy cows is still limited, and the specific regulatory mechanism is still unknown. In this study, we used primary bovine mammary epithelial cells(BMECs)as a model and utilized overexpression and knockdown techniques for the PI4K2A gene to investigate the specific mechanisms by which it regulates lipid metabolism in BMECs. We studied whether PI4K2A regulates the inhibition of trans-10, cis-12 conjugated linoleic acid (t10,c12-CLA) on lipid synthesis in BMECs. The results revealed that PI4K2A plays a crucial regulatory role within the cytoplasm of BMECs, positively impacting fatty acid (FA) transport, triacylglycerol (TAG) synthesis, and lipid droplet secretion (p < 0.05 for all effects). Furthermore, PI4K2A promotes cell proliferation and inhibits apoptosis (p < 0.05). This study also confirmed that overexpressing the PI4K2A gene can effectively reduce the inhibitory effect of t10,c12-CLA on lipid synthesis in BMECs. Our findings have deepened the understanding of the regulatory mechanisms of milk fat synthesis and provide potential genetic targets for the improvement of lactation traits in dairy cows. |
| format | Article |
| id | doaj-art-80a165a592f646c199120bedac6c67f1 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-80a165a592f646c199120bedac6c67f12025-02-02T12:16:17ZengNature PortfolioScientific Reports2045-23222025-01-0115111610.1038/s41598-025-87089-2The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesisTong Mu0Honghong Hu1Xiaofang Feng2Chuanchuan Wang3Juan Zhang4Zhihong Sun5Yaling Gu6School of Life Science, Yan’an UniversitySchool of Life Science, Yan’an UniversityCollege of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia UniversityCollege of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia UniversityCollege of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia UniversitySchool of Life Science, Yan’an UniversityCollege of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia UniversityAbstract Currently, the identification of valuable candidate genes affecting milk fat synthesis in dairy cows is still limited, and the specific regulatory mechanism is still unknown. In this study, we used primary bovine mammary epithelial cells(BMECs)as a model and utilized overexpression and knockdown techniques for the PI4K2A gene to investigate the specific mechanisms by which it regulates lipid metabolism in BMECs. We studied whether PI4K2A regulates the inhibition of trans-10, cis-12 conjugated linoleic acid (t10,c12-CLA) on lipid synthesis in BMECs. The results revealed that PI4K2A plays a crucial regulatory role within the cytoplasm of BMECs, positively impacting fatty acid (FA) transport, triacylglycerol (TAG) synthesis, and lipid droplet secretion (p < 0.05 for all effects). Furthermore, PI4K2A promotes cell proliferation and inhibits apoptosis (p < 0.05). This study also confirmed that overexpressing the PI4K2A gene can effectively reduce the inhibitory effect of t10,c12-CLA on lipid synthesis in BMECs. Our findings have deepened the understanding of the regulatory mechanisms of milk fat synthesis and provide potential genetic targets for the improvement of lactation traits in dairy cows.https://doi.org/10.1038/s41598-025-87089-2Holstein cattleMilk fatPI4K2At10,c12-CLARegulation mechanism |
| spellingShingle | Tong Mu Honghong Hu Xiaofang Feng Chuanchuan Wang Juan Zhang Zhihong Sun Yaling Gu The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis Scientific Reports Holstein cattle Milk fat PI4K2A t10,c12-CLA Regulation mechanism |
| title | The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis |
| title_full | The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis |
| title_fullStr | The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis |
| title_full_unstemmed | The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis |
| title_short | The PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis |
| title_sort | pi4k2a gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10 c12 cla on lipid synthesis |
| topic | Holstein cattle Milk fat PI4K2A t10,c12-CLA Regulation mechanism |
| url | https://doi.org/10.1038/s41598-025-87089-2 |
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