Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1
To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cɛ mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immu...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/68069 |
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| author | Kazuhiko Hanashiro Shigeto Ohta Masanori Sunagawa Mariko Nakamura Mikio Suzuki Tadayoshi Kosugi |
| author_facet | Kazuhiko Hanashiro Shigeto Ohta Masanori Sunagawa Mariko Nakamura Mikio Suzuki Tadayoshi Kosugi |
| author_sort | Kazuhiko Hanashiro |
| collection | DOAJ |
| description | To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by
Epstein-Barr virus (EBV) on Cɛ mRNA expression,
mRNA levels were examined by RT-PCR
or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from
Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris
suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1,
using lipofection method. Cɛ mRNA levels were
considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from
the nonimmunized rats; however, Cɛ mRNA levels were
decreased in the splenocytes derived from the immunized rats. Cɛ
mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on
the differentiation status of B cells upon exposure to allergen. |
| format | Article |
| id | doaj-art-809cc892179c4aa5b0010fe1aa2a734a |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-809cc892179c4aa5b0010fe1aa2a734a2025-02-03T01:21:10ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/6806968069Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1Kazuhiko Hanashiro0Shigeto Ohta1Masanori Sunagawa2Mariko Nakamura3Mikio Suzuki4Tadayoshi Kosugi51st Department of Physiology, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan1st Department of Physiology, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan1st Department of Physiology, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan1st Department of Physiology, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanTo evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cɛ mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1, using lipofection method. Cɛ mRNA levels were considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from the nonimmunized rats; however, Cɛ mRNA levels were decreased in the splenocytes derived from the immunized rats. Cɛ mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on the differentiation status of B cells upon exposure to allergen.http://dx.doi.org/10.1155/MI/2006/68069 |
| spellingShingle | Kazuhiko Hanashiro Shigeto Ohta Masanori Sunagawa Mariko Nakamura Mikio Suzuki Tadayoshi Kosugi Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 Mediators of Inflammation |
| title | Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 |
| title_full | Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 |
| title_fullStr | Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 |
| title_full_unstemmed | Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 |
| title_short | Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1 |
| title_sort | modification of ce mrna expression by ebv encoded latent membrane protein 1 |
| url | http://dx.doi.org/10.1155/MI/2006/68069 |
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