Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1
To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cɛ mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immu...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/68069 |
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| Summary: | To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by
Epstein-Barr virus (EBV) on Cɛ mRNA expression,
mRNA levels were examined by RT-PCR
or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from
Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris
suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1,
using lipofection method. Cɛ mRNA levels were
considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from
the nonimmunized rats; however, Cɛ mRNA levels were
decreased in the splenocytes derived from the immunized rats. Cɛ
mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on
the differentiation status of B cells upon exposure to allergen. |
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| ISSN: | 0962-9351 1466-1861 |