Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice
Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. ang...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2019/5979546 |
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| author | Awol Mekonnen Solomon Tesfaye Selam G. Christos Kassahun Dires Tizazu Zenebe Nigus Zegeye Yoseph Shiferaw Ermias Lulekal |
| author_facet | Awol Mekonnen Solomon Tesfaye Selam G. Christos Kassahun Dires Tizazu Zenebe Nigus Zegeye Yoseph Shiferaw Ermias Lulekal |
| author_sort | Awol Mekonnen |
| collection | DOAJ |
| description | Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic. |
| format | Article |
| id | doaj-art-807d19d684514820851846085dd0ec57 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-807d19d684514820851846085dd0ec572025-02-03T05:57:12ZengWileyJournal of Toxicology1687-81911687-82052019-01-01201910.1155/2019/59795465979546Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and MiceAwol Mekonnen0Solomon Tesfaye1Selam G. Christos2Kassahun Dires3Tizazu Zenebe4Nigus Zegeye5Yoseph Shiferaw6Ermias Lulekal7College of Medicine, Institute of Health Science and Medicine, Debre-Berhan Universty, P.O. Box 445, Debre-Berhan, EthiopiaSchool of Medicine, Institute of Health Science and Medicine, Jijiga University, P.O. Box, 1020, Jijiga, EthiopiaCollege of Medicine, Institute of Health Science and Medicine, Debre-Berhan Universty, P.O. Box 445, Debre-Berhan, EthiopiaCollege of Medicine, Institute of Health Science and Medicine, Debre-Berhan Universty, P.O. Box 445, Debre-Berhan, EthiopiaCollege of Medicine, Institute of Health Science and Medicine, Debre-Berhan Universty, P.O. Box 445, Debre-Berhan, EthiopiaCollege of Medicine, Institute of Health Science and Medicine, Debre-Berhan Universty, P.O. Box 445, Debre-Berhan, EthiopiaChemistry Department, College Computational Sciences and of Natural, Debre-Berhan University, P.O. Box 445, Debre-Berhan, EthiopiaPlant Biology and Biodiversity Management Department, College of Computational Sciences and Natural, Addis Ababa University, Private P.O. Box 34731, Addis Ababa, EthiopiaLavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic.http://dx.doi.org/10.1155/2019/5979546 |
| spellingShingle | Awol Mekonnen Solomon Tesfaye Selam G. Christos Kassahun Dires Tizazu Zenebe Nigus Zegeye Yoseph Shiferaw Ermias Lulekal Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice Journal of Toxicology |
| title | Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice |
| title_full | Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice |
| title_fullStr | Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice |
| title_full_unstemmed | Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice |
| title_short | Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice |
| title_sort | evaluation of skin irritation and acute and subacute oral toxicity of lavandula angustifolia essential oils in rabbit and mice |
| url | http://dx.doi.org/10.1155/2019/5979546 |
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