Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration
The aim of this study was to evaluate the effect of intra-articular (IA) or a combination of intra-articular and intraosseous (IO) infiltration of Platelet Rich Plasma (PRP) on the cellular content of synovial fluid (SF) of osteoarthritic patients. Thirty-one patients received a single infiltration...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1247950 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558459912978432 |
|---|---|
| author | Emma Muiños-López Diego Delgado Pello Sánchez Bruno Paiva Eduardo Anitua Nicolás Fiz Beatriz Aizpurua Jorge Guadilla Sabino Padilla Froilán Granero-Moltó Felipe Prósper Mikel Sánchez |
| author_facet | Emma Muiños-López Diego Delgado Pello Sánchez Bruno Paiva Eduardo Anitua Nicolás Fiz Beatriz Aizpurua Jorge Guadilla Sabino Padilla Froilán Granero-Moltó Felipe Prósper Mikel Sánchez |
| author_sort | Emma Muiños-López |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of intra-articular (IA) or a combination of intra-articular and intraosseous (IO) infiltration of Platelet Rich Plasma (PRP) on the cellular content of synovial fluid (SF) of osteoarthritic patients. Thirty-one patients received a single infiltration of PRP either in the IA space (n=14) or in the IA space together with two IO infiltrations, one in the medial femoral condyle and one in the tibial plateau (n=17). SF was collected before and after one week of the infiltration. The presence in the SF of mesenchymal stem cells (MSCs), monocytes, and lymphocytes was determined and quantified by flow cytometry. The number and identity of the MSCs were further confirmed by colony-forming and differentiation assays. PRP infiltration into the subchondral bone (SB) and the IA space induced a reduction in the population of MSCs in the SF. This reduction in MSCs was further confirmed by colony-forming (CFU-F) assay. On the contrary, IA infiltration alone did not cause variations in any of the cellular populations by flow cytometry or CFU-F assay. The SF of osteoarthritic patients contains a population of MSCs that can be modulated by PRP infiltration of the SB compartment. |
| format | Article |
| id | doaj-art-8070307904784bfab8e01473f57d6b6e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8070307904784bfab8e01473f57d6b6e2025-02-03T01:32:15ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/12479501247950Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma AdministrationEmma Muiños-López0Diego Delgado1Pello Sánchez2Bruno Paiva3Eduardo Anitua4Nicolás Fiz5Beatriz Aizpurua6Jorge Guadilla7Sabino Padilla8Froilán Granero-Moltó9Felipe Prósper10Mikel Sánchez11Cell Therapy Area, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, SpainArthroscopic Surgery Unit Research, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainArthroscopic Surgery Unit Research, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainCenter for Applied Medical Research, Av. de Pío XII 55, 31008 Pamplona, SpainFundacion Eduardo Anitua, C/Jose María Cagigal 19, 01007 Vitoria-Gasteiz, SpainArthroscopic Surgery Unit, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainArthroscopic Surgery Unit, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainArthroscopic Surgery Unit, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainFundacion Eduardo Anitua, C/Jose María Cagigal 19, 01007 Vitoria-Gasteiz, SpainCell Therapy Area, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, SpainCell Therapy Area, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, SpainArthroscopic Surgery Unit Research, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, SpainThe aim of this study was to evaluate the effect of intra-articular (IA) or a combination of intra-articular and intraosseous (IO) infiltration of Platelet Rich Plasma (PRP) on the cellular content of synovial fluid (SF) of osteoarthritic patients. Thirty-one patients received a single infiltration of PRP either in the IA space (n=14) or in the IA space together with two IO infiltrations, one in the medial femoral condyle and one in the tibial plateau (n=17). SF was collected before and after one week of the infiltration. The presence in the SF of mesenchymal stem cells (MSCs), monocytes, and lymphocytes was determined and quantified by flow cytometry. The number and identity of the MSCs were further confirmed by colony-forming and differentiation assays. PRP infiltration into the subchondral bone (SB) and the IA space induced a reduction in the population of MSCs in the SF. This reduction in MSCs was further confirmed by colony-forming (CFU-F) assay. On the contrary, IA infiltration alone did not cause variations in any of the cellular populations by flow cytometry or CFU-F assay. The SF of osteoarthritic patients contains a population of MSCs that can be modulated by PRP infiltration of the SB compartment.http://dx.doi.org/10.1155/2016/1247950 |
| spellingShingle | Emma Muiños-López Diego Delgado Pello Sánchez Bruno Paiva Eduardo Anitua Nicolás Fiz Beatriz Aizpurua Jorge Guadilla Sabino Padilla Froilán Granero-Moltó Felipe Prósper Mikel Sánchez Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration Stem Cells International |
| title | Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration |
| title_full | Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration |
| title_fullStr | Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration |
| title_full_unstemmed | Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration |
| title_short | Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration |
| title_sort | modulation of synovial fluid derived mesenchymal stem cells by intra articular and intraosseous platelet rich plasma administration |
| url | http://dx.doi.org/10.1155/2016/1247950 |
| work_keys_str_mv | AT emmamuinoslopez modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT diegodelgado modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT pellosanchez modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT brunopaiva modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT eduardoanitua modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT nicolasfiz modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT beatrizaizpurua modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT jorgeguadilla modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT sabinopadilla modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT froilangraneromolto modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT felipeprosper modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration AT mikelsanchez modulationofsynovialfluidderivedmesenchymalstemcellsbyintraarticularandintraosseousplateletrichplasmaadministration |