Effect of Vitamin C, Alpha Lipoic Acid (ALA), and Pentoxifylline on the Sperm Parameters, Cryosurvival Rate, and MDA Concentration after Thawing in Cryopreservation
Cryopreservation provides available sperm for subsequent use in Assisted Reproductive Technology (ART), but it still has detrimental effects on sperm quality. Although several investigations of the use of antioxidants in cryopreservation mediums had conducted, the result remains unsatisfactory. Ther...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2025-01-01
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| Series: | International Journal of Technology |
| Subjects: | |
| Online Access: | https://ijtech.eng.ui.ac.id/article/view/6305 |
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| Summary: | Cryopreservation provides available sperm for subsequent use in Assisted Reproductive Technology (ART), but it still has detrimental effects on sperm quality. Although several investigations of the use of antioxidants in cryopreservation mediums had conducted, the result remains unsatisfactory. Therefore, this study aimed to contribute to the references of the antioxidant role in preserving the quality of sperm in cryopreservation by examining post-thawing sperm quality, cryosurvival rate, and post-thawing malondialdehyde (MDA) concentration. The semen sample of 35 infertile patients was classified as normozoospermic and non-normozoospermic. The semen sample was divided into four aliquots, with the following antioxidants supplemented to cryomedium: vitamin C (Vit C), alpha lipoic acid (ALA), and pentoxifylline (PTX). Subsequently, it was cryopreserved in liquid nitrogen, and the parameters: sperm quality (concentration, motility, and morphology), cryosurvival rate, and MDA concentration were evaluated after thawing. All parameters showed a reduction in post-thawing analysis. PTX group significantly improves sperm progressive motility and cryosurvival rate (CSR) in normozoospermic (42.71 ± 4.30% and 78.54 ± 6.62%) among other treatments. The ALA and PTX groups decreased MDA concentration in normozoospermic, while Vit C showed a decrease in non-normozoospermic as well. We assumed that the antioxidants used in this study differently improved the sperm quality parameter post-thawing in different types of sperm abnormalities, such as PTX optimally increasing sperm motility after thawing, even though all antioxidants are likely to suppress MDA concentration after thawing.. These interventions may be beneficial in improving thawed sperm quality to improve ART program success. |
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| ISSN: | 2086-9614 2087-2100 |