Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics
Silica–poly (vinylpyrrolidone) hybrid material was prepared using the sol–gel method. Tetramethyl ortosilane (TMOS) was used as a silica precursor. XRD analysis established that the as-prepared material is amorphous. The morphological structure of the final product was determined by the incorporated...
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MDPI AG
2024-11-01
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| author | Lilia Yordanova Yoanna Kostova Elitsa Pavlova Albena Bachvarova-Nedelcheva Iliana Ivanova Elena Nenova |
| author_facet | Lilia Yordanova Yoanna Kostova Elitsa Pavlova Albena Bachvarova-Nedelcheva Iliana Ivanova Elena Nenova |
| author_sort | Lilia Yordanova |
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| description | Silica–poly (vinylpyrrolidone) hybrid material was prepared using the sol–gel method. Tetramethyl ortosilane (TMOS) was used as a silica precursor. XRD analysis established that the as-prepared material is amorphous. The morphological structure of the final product was determined by the incorporated PVP. The UV–Vis analysis showed that the obtained hybrid exhibited absorption in the ultraviolet range. The antimicrobial activity of the SiO<sub>2</sub>/15PVP hybrid material was tested on <i>Staphylococcus epidermidis</i> ATCC 14990, <i>Salmonella typhimurium</i> ATCC BAA-2162, <i>Candida albicans</i>, and <i>Saccharomyces cerevisiae</i> in combination with the following antibiotics: Vancomycin for Gram-positive bacteria, Ciprofloxacin for Gram-negative bacteria, and Nystatin for yeast. The results confirmed a concentration-dependent synergistic effect of the antibiotic in combination with the TM15/PVP hybrid particles, especially at their highest concentration of 100 mg/mL on Gram-positive bacteria and for the Gram-negative <i>Salmonella</i>. On <i>Candida albicans</i> ATCC 18804 and <i>Saccharomyces cerevisiae</i> CCY 21-6-3, the effect was synergistic again, and a fungicidal effect was observed at 6.25 and 1.50 mg/mL for the antibiotic concentration and concentrations of hybrid material at 100 mg/mL. The toxicity on <i>Daphnia magna</i> was also tested. The registered prooxidant activity of SiO<sub>2</sub>/15PVP shows possible applications at very low concentrations. The obtained results demonstrate the possibility of clinical implementations of the newly synthesized hybrid material. |
| format | Article |
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| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-8005a2da2ca04bc5ba5b1dd7dbdf5efa2025-08-20T02:48:01ZengMDPI AGMolecules1420-30492024-11-012922532210.3390/molecules29225322Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with AntibioticsLilia Yordanova0Yoanna Kostova1Elitsa Pavlova2Albena Bachvarova-Nedelcheva3Iliana Ivanova4Elena Nenova5Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaInstitute of Metal Science, Equipment and Technologies with Hydro- and Aerodynamics Centre “Acad. A. Balevski”, Bulgarian Academy of Sciences, Shipchenski Prohod Str., 67, 1574 Sofia, BulgariaFaculty of Physics, Sofia University “St. Kliment Ohridski”, 5 James Boucher Blvd., 1164 Sofia, BulgariaInstitute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 11, 1113 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaSilica–poly (vinylpyrrolidone) hybrid material was prepared using the sol–gel method. Tetramethyl ortosilane (TMOS) was used as a silica precursor. XRD analysis established that the as-prepared material is amorphous. The morphological structure of the final product was determined by the incorporated PVP. The UV–Vis analysis showed that the obtained hybrid exhibited absorption in the ultraviolet range. The antimicrobial activity of the SiO<sub>2</sub>/15PVP hybrid material was tested on <i>Staphylococcus epidermidis</i> ATCC 14990, <i>Salmonella typhimurium</i> ATCC BAA-2162, <i>Candida albicans</i>, and <i>Saccharomyces cerevisiae</i> in combination with the following antibiotics: Vancomycin for Gram-positive bacteria, Ciprofloxacin for Gram-negative bacteria, and Nystatin for yeast. The results confirmed a concentration-dependent synergistic effect of the antibiotic in combination with the TM15/PVP hybrid particles, especially at their highest concentration of 100 mg/mL on Gram-positive bacteria and for the Gram-negative <i>Salmonella</i>. On <i>Candida albicans</i> ATCC 18804 and <i>Saccharomyces cerevisiae</i> CCY 21-6-3, the effect was synergistic again, and a fungicidal effect was observed at 6.25 and 1.50 mg/mL for the antibiotic concentration and concentrations of hybrid material at 100 mg/mL. The toxicity on <i>Daphnia magna</i> was also tested. The registered prooxidant activity of SiO<sub>2</sub>/15PVP shows possible applications at very low concentrations. The obtained results demonstrate the possibility of clinical implementations of the newly synthesized hybrid material.https://www.mdpi.com/1420-3049/29/22/5322hybridoptical propertiesantibacterial propertiesantibioticsprooxidant propertiesenvironmental toxicity control |
| spellingShingle | Lilia Yordanova Yoanna Kostova Elitsa Pavlova Albena Bachvarova-Nedelcheva Iliana Ivanova Elena Nenova Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics Molecules hybrid optical properties antibacterial properties antibiotics prooxidant properties environmental toxicity control |
| title | Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics |
| title_full | Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics |
| title_fullStr | Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics |
| title_full_unstemmed | Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics |
| title_short | Optical Properties and Antimicrobial Activity of Si/PVP Hybrid Material Combined with Antibiotics |
| title_sort | optical properties and antimicrobial activity of si pvp hybrid material combined with antibiotics |
| topic | hybrid optical properties antibacterial properties antibiotics prooxidant properties environmental toxicity control |
| url | https://www.mdpi.com/1420-3049/29/22/5322 |
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