Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism
Mast cells are primary effector cells of allergy, and recruitment of mast cells in involved tissue is one of the key events in allergic inflammation. Tryptase is the most abundant secretory product of mast cells, but little is known of its influence on mast cell accumulation. Using mouse peritoneal...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/6431574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564129667219456 |
|---|---|
| author | Xin Liu Junling Wang Huiyun Zhang Mengmeng Zhan Hanqiu Chen Zeman Fang Chiyan Xu Huifang Chen Shaoheng He |
| author_facet | Xin Liu Junling Wang Huiyun Zhang Mengmeng Zhan Hanqiu Chen Zeman Fang Chiyan Xu Huifang Chen Shaoheng He |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Mast cells are primary effector cells of allergy, and recruitment of mast cells in involved tissue is one of the key events in allergic inflammation. Tryptase is the most abundant secretory product of mast cells, but little is known of its influence on mast cell accumulation. Using mouse peritoneal model, cell migration assay, and flow cytometry analysis, we investigated role of tryptase in recruiting mast cells. The results showed that tryptase induced up to 6.7-fold increase in mast cell numbers in mouse peritoneum following injection. Inhibitors of tryptase, an antagonist of PAR-2 FSLLRY-NH2, and pretreatment of mice with anti-ICAM-1, anti-CD11a, and anti-CD18 antibodies dramatically diminished tryptase induced mast cell accumulation. On the other hand, PAR-2 agonist peptides SLIGRL-NH2 and tc-LIGRLO-NH2 provoked mast cell accumulation following injection. These implicate that tryptase induced mast cell accumulation is dependent on its enzymatic activity, activation of PAR-2, and interaction between ICAM-1 and LFA-1. Moreover, induction of trans-endothelium migration of mast cells in vitro indicates that tryptase acts as a chemoattractant. In conclusion, provocation of mast cell accumulation by mast cell tryptase suggests a novel self-amplification mechanism of mast cell accumulation. Mast cell stabilizers as well as PAR-2 antagonist agents may be useful for treatment of allergic reactions. |
| format | Article |
| id | doaj-art-7ffe23cf9cda4045b27447704c13390d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7ffe23cf9cda4045b27447704c13390d2025-02-03T01:11:45ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/64315746431574Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent MechanismXin Liu0Junling Wang1Huiyun Zhang2Mengmeng Zhan3Hanqiu Chen4Zeman Fang5Chiyan Xu6Huifang Chen7Shaoheng He8Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaAllergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaAllergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaAllergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaAllergy and Inflammation Research Institute, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, ChinaAllergy and Inflammation Research Institute, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, ChinaAllergy and Inflammation Research Institute, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, ChinaAllergy and Inflammation Research Institute, The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, ChinaAllergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaMast cells are primary effector cells of allergy, and recruitment of mast cells in involved tissue is one of the key events in allergic inflammation. Tryptase is the most abundant secretory product of mast cells, but little is known of its influence on mast cell accumulation. Using mouse peritoneal model, cell migration assay, and flow cytometry analysis, we investigated role of tryptase in recruiting mast cells. The results showed that tryptase induced up to 6.7-fold increase in mast cell numbers in mouse peritoneum following injection. Inhibitors of tryptase, an antagonist of PAR-2 FSLLRY-NH2, and pretreatment of mice with anti-ICAM-1, anti-CD11a, and anti-CD18 antibodies dramatically diminished tryptase induced mast cell accumulation. On the other hand, PAR-2 agonist peptides SLIGRL-NH2 and tc-LIGRLO-NH2 provoked mast cell accumulation following injection. These implicate that tryptase induced mast cell accumulation is dependent on its enzymatic activity, activation of PAR-2, and interaction between ICAM-1 and LFA-1. Moreover, induction of trans-endothelium migration of mast cells in vitro indicates that tryptase acts as a chemoattractant. In conclusion, provocation of mast cell accumulation by mast cell tryptase suggests a novel self-amplification mechanism of mast cell accumulation. Mast cell stabilizers as well as PAR-2 antagonist agents may be useful for treatment of allergic reactions.http://dx.doi.org/10.1155/2016/6431574 |
| spellingShingle | Xin Liu Junling Wang Huiyun Zhang Mengmeng Zhan Hanqiu Chen Zeman Fang Chiyan Xu Huifang Chen Shaoheng He Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism Mediators of Inflammation |
| title | Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism |
| title_full | Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism |
| title_fullStr | Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism |
| title_full_unstemmed | Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism |
| title_short | Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism |
| title_sort | induction of mast cell accumulation by tryptase via a protease activated receptor 2 and icam 1 dependent mechanism |
| url | http://dx.doi.org/10.1155/2016/6431574 |
| work_keys_str_mv | AT xinliu inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT junlingwang inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT huiyunzhang inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT mengmengzhan inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT hanqiuchen inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT zemanfang inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT chiyanxu inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT huifangchen inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism AT shaohenghe inductionofmastcellaccumulationbytryptaseviaaproteaseactivatedreceptor2andicam1dependentmechanism |