Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application
Interpolyelectrolyte complexes (IPECs) of polysaccharides are multifunctional polymer materials that improve the mechanical and physicochemical properties of individual polysaccharides. In this study, highly porous (>90%) materials based on IPECs of versatile natural polysaccharides, chitosan (30...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Advances in Polymer Technology |
| Online Access: | http://dx.doi.org/10.1155/2024/8747902 |
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| author | Aliaksandr Kraskouski Maksim Mashkin Viktoryia Kulikouskaya Viktoryia Savich Anastasiya Sidarenka Sergei Pinchuk Ruibin Li |
| author_facet | Aliaksandr Kraskouski Maksim Mashkin Viktoryia Kulikouskaya Viktoryia Savich Anastasiya Sidarenka Sergei Pinchuk Ruibin Li |
| author_sort | Aliaksandr Kraskouski |
| collection | DOAJ |
| description | Interpolyelectrolyte complexes (IPECs) of polysaccharides are multifunctional polymer materials that improve the mechanical and physicochemical properties of individual polysaccharides. In this study, highly porous (>90%) materials based on IPECs of versatile natural polysaccharides, chitosan (30 and 1,200 kDa) and pectin, are obtained by freeze-drying technique. To enhance the interaction between chitosan and pectin macromolecules, the latter are chemically functionalized with dialdehyde groups. The chitosan-/aldehyde-functionalized pectin (Chit/AF-Pect) polyelectrolyte complex sponges obtained are characterized using SEM, FTIR spectroscopy, and TGA. The swelling capacity study reveals a higher swelling ratio of IPEC sponges with an increase in both the molecular weight and content of chitosan: for Chit30/AF-Pect, the swelling ratio rises from 327% to 480%, while for Chit1200/AF-Pect, from 681% to 1,066%. Additionally, the in vitro degradation test demonstrates higher stability of Chit1200/AF-Pect sponges in comparison with those of Chit30/AF-Pect: after 4 days of incubation, the weight losses are found to be 9%–16% and 18%–41%, respectively. The cytotoxicity study shows that Chit30/AF-Pect sponges are noncytotoxic, with cell viability values >70%. Furthermore, the Chit30/AF-Pect sponges, obtained at chitosan:pectin weight ratio of 5:1, exhibit bactericidal activity against Escherichia coli BIM B-984 G, Pseudomonas aeruginosa BIM B-807 G, Staphylococcus aureus BIM B-1841, and slightly inhibit the growth of Enterococcus faecalis BIM B-1530 G. These findings indicate that the obtained Chit30/AF-Pect sponges can be used to create wound dressings for wound healing applications. |
| format | Article |
| id | doaj-art-7fc1bcf39efd4671932fb0505b62b3cd |
| institution | Kabale University |
| issn | 1098-2329 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Polymer Technology |
| spelling | doaj-art-7fc1bcf39efd4671932fb0505b62b3cd2025-02-03T07:23:24ZengWileyAdvances in Polymer Technology1098-23292024-01-01202410.1155/2024/8747902Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing ApplicationAliaksandr Kraskouski0Maksim Mashkin1Viktoryia Kulikouskaya2Viktoryia Savich3Anastasiya Sidarenka4Sergei Pinchuk5Ruibin Li6Institute of Chemistry of New Materials of the National Academy of Sciences of BelarusInstitute of Chemistry of New Materials of the National Academy of Sciences of BelarusInstitute of Chemistry of New Materials of the National Academy of Sciences of BelarusInstitute of Microbiology of the National Academy of Sciences of BelarusInstitute of Microbiology of the National Academy of Sciences of BelarusInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of BelarusState Key Laboratory of Radiation Medicine and ProtectionInterpolyelectrolyte complexes (IPECs) of polysaccharides are multifunctional polymer materials that improve the mechanical and physicochemical properties of individual polysaccharides. In this study, highly porous (>90%) materials based on IPECs of versatile natural polysaccharides, chitosan (30 and 1,200 kDa) and pectin, are obtained by freeze-drying technique. To enhance the interaction between chitosan and pectin macromolecules, the latter are chemically functionalized with dialdehyde groups. The chitosan-/aldehyde-functionalized pectin (Chit/AF-Pect) polyelectrolyte complex sponges obtained are characterized using SEM, FTIR spectroscopy, and TGA. The swelling capacity study reveals a higher swelling ratio of IPEC sponges with an increase in both the molecular weight and content of chitosan: for Chit30/AF-Pect, the swelling ratio rises from 327% to 480%, while for Chit1200/AF-Pect, from 681% to 1,066%. Additionally, the in vitro degradation test demonstrates higher stability of Chit1200/AF-Pect sponges in comparison with those of Chit30/AF-Pect: after 4 days of incubation, the weight losses are found to be 9%–16% and 18%–41%, respectively. The cytotoxicity study shows that Chit30/AF-Pect sponges are noncytotoxic, with cell viability values >70%. Furthermore, the Chit30/AF-Pect sponges, obtained at chitosan:pectin weight ratio of 5:1, exhibit bactericidal activity against Escherichia coli BIM B-984 G, Pseudomonas aeruginosa BIM B-807 G, Staphylococcus aureus BIM B-1841, and slightly inhibit the growth of Enterococcus faecalis BIM B-1530 G. These findings indicate that the obtained Chit30/AF-Pect sponges can be used to create wound dressings for wound healing applications.http://dx.doi.org/10.1155/2024/8747902 |
| spellingShingle | Aliaksandr Kraskouski Maksim Mashkin Viktoryia Kulikouskaya Viktoryia Savich Anastasiya Sidarenka Sergei Pinchuk Ruibin Li Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application Advances in Polymer Technology |
| title | Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application |
| title_full | Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application |
| title_fullStr | Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application |
| title_full_unstemmed | Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application |
| title_short | Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application |
| title_sort | design of highly porous materials based on chitosan pectin interpolyelectrolyte complex for wound healing application |
| url | http://dx.doi.org/10.1155/2024/8747902 |
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