Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep
The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Ex...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/147158 |
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| author | Ana Cristina Pérez de Diego Pedro José Sánchez-Cordón Ana Isabel de las Heras José Manuel Sánchez-Vizcaíno |
| author_facet | Ana Cristina Pérez de Diego Pedro José Sánchez-Cordón Ana Isabel de las Heras José Manuel Sánchez-Vizcaíno |
| author_sort | Ana Cristina Pérez de Diego |
| collection | DOAJ |
| description | The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4+, CD8+, and WC1+), CD25+ regulatory cells, or CD14+ monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14+ monocytes, CD25+ regulatory cells, and CD8+ cytotoxic T lymphocytes. |
| format | Article |
| id | doaj-art-7fbb49ae78d24cc38f04903d9b9d8263 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7fbb49ae78d24cc38f04903d9b9d82632025-02-03T01:11:10ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/147158147158Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in SheepAna Cristina Pérez de Diego0Pedro José Sánchez-Cordón1Ana Isabel de las Heras2José Manuel Sánchez-Vizcaíno3VISAVET Health Surveillance Centre and Animal Health Department, Veterinary Faculty, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainDepartment of Comparative Pathology, Veterinary Faculty, University of Córdoba, Animal Health Building, Rabanales Campus, 14014 Córdoba, SpainVISAVET Health Surveillance Centre and Animal Health Department, Veterinary Faculty, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre and Animal Health Department, Veterinary Faculty, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainThe protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4+, CD8+, and WC1+), CD25+ regulatory cells, or CD14+ monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14+ monocytes, CD25+ regulatory cells, and CD8+ cytotoxic T lymphocytes.http://dx.doi.org/10.1100/2012/147158 |
| spellingShingle | Ana Cristina Pérez de Diego Pedro José Sánchez-Cordón Ana Isabel de las Heras José Manuel Sánchez-Vizcaíno Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep The Scientific World Journal |
| title | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
| title_full | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
| title_fullStr | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
| title_full_unstemmed | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
| title_short | Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep |
| title_sort | characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep |
| url | http://dx.doi.org/10.1100/2012/147158 |
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