Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period
Abstract Background Thiol/disulphide homeostasis (TDH) has a critical role in many cellular activities such as antioxidant protection. Alterations of oxidative stress in the transition period play an important role in development of some diseases and disorders in dairy cows. Objectives The purpose o...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70023 |
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| author | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Guzin Ozkurt Ali Burak Balkan Tevhide Sel |
| author_facet | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Guzin Ozkurt Ali Burak Balkan Tevhide Sel |
| author_sort | Burcu Menekse Balkan |
| collection | DOAJ |
| description | Abstract Background Thiol/disulphide homeostasis (TDH) has a critical role in many cellular activities such as antioxidant protection. Alterations of oxidative stress in the transition period play an important role in development of some diseases and disorders in dairy cows. Objectives The purpose of this study is to assess the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total thiol, native thiol, disulphide and lipid profile in Simmental and Montofon dairy cows (aged 2–3) before and after calving. Methods Blood samples were collected 233–280 days of pregnancy and the 30 days of post‐partum. Serum total thiol, native thiol and disulphide levels were determined as well as TAS, TOS and paraoxonase‐1 (PON‐1) levels were measured using colorimetric assays. Triglycerides (TG), total cholesterol, HDL cholesterol and LDL cholesterol levels were measured with an automatic analyser. Results Total thiol (p = 0.038) and disulphide (p = 0.015) levels were higher after calving compare to pregnancy in Montofon. TAS was found lower (p < 0.001), and OSI was higher in both breeds (Montofon p = 0.012, Simmental p = 0.028) after calving than in pregnancy. When compared between pregnancy and after calving levels in the same breed, HDL was found to be higher after calving (p < 0.001) and TG was lower after calving (p = 0.020) in Montofon. PON (p = 0.090), HDL (p < 0.001) and cholesterol levels were found higher (p < 0.001) and TG level was lower (p < 0.001) after calving in Simmental. Conclusions According to our results, we observed different responses between two breeds before and after calving. There are few studies about TDH in animal research, and this is the first study in the literature that evaluates the TDH along with oxidative stress and lipid profiles in dairy cows in the periparturient and post‐partum period. |
| format | Article |
| id | doaj-art-7fa5bead70364c76baff262daa145688 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-7fa5bead70364c76baff262daa1456882025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70023Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum periodBurcu Menekse Balkan0Ogunc Meral1Bekir Cetintav2Guzin Ozkurt3Ali Burak Balkan4Tevhide Sel5Department of Biochemistry, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Biochemistry, Faculty of Veterinary Medicine Ankara University Ankara TurkeyDepartment of Biostatistics, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Biochemistry, Faculty of Veterinary Medicine Aksaray University Aksaray TurkeyRepublic of Turkiye Ministry of Agriculture and Forestry Burdur TurkeyDepartment of Biochemistry, Faculty of Veterinary Medicine Ankara University Ankara TurkeyAbstract Background Thiol/disulphide homeostasis (TDH) has a critical role in many cellular activities such as antioxidant protection. Alterations of oxidative stress in the transition period play an important role in development of some diseases and disorders in dairy cows. Objectives The purpose of this study is to assess the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total thiol, native thiol, disulphide and lipid profile in Simmental and Montofon dairy cows (aged 2–3) before and after calving. Methods Blood samples were collected 233–280 days of pregnancy and the 30 days of post‐partum. Serum total thiol, native thiol and disulphide levels were determined as well as TAS, TOS and paraoxonase‐1 (PON‐1) levels were measured using colorimetric assays. Triglycerides (TG), total cholesterol, HDL cholesterol and LDL cholesterol levels were measured with an automatic analyser. Results Total thiol (p = 0.038) and disulphide (p = 0.015) levels were higher after calving compare to pregnancy in Montofon. TAS was found lower (p < 0.001), and OSI was higher in both breeds (Montofon p = 0.012, Simmental p = 0.028) after calving than in pregnancy. When compared between pregnancy and after calving levels in the same breed, HDL was found to be higher after calving (p < 0.001) and TG was lower after calving (p = 0.020) in Montofon. PON (p = 0.090), HDL (p < 0.001) and cholesterol levels were found higher (p < 0.001) and TG level was lower (p < 0.001) after calving in Simmental. Conclusions According to our results, we observed different responses between two breeds before and after calving. There are few studies about TDH in animal research, and this is the first study in the literature that evaluates the TDH along with oxidative stress and lipid profiles in dairy cows in the periparturient and post‐partum period.https://doi.org/10.1002/vms3.70023antioxidant statusdairy cowslipid profilesperiparturient periodpost‐partum periodthiol‐disulphide homeostasis |
| spellingShingle | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Guzin Ozkurt Ali Burak Balkan Tevhide Sel Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period Veterinary Medicine and Science antioxidant status dairy cows lipid profiles periparturient period post‐partum period thiol‐disulphide homeostasis |
| title | Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period |
| title_full | Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period |
| title_fullStr | Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period |
| title_full_unstemmed | Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period |
| title_short | Evaluation of thiol/disulphide and oxidant–antioxidant status of dairy cows in periparturient and post‐partum period |
| title_sort | evaluation of thiol disulphide and oxidant antioxidant status of dairy cows in periparturient and post partum period |
| topic | antioxidant status dairy cows lipid profiles periparturient period post‐partum period thiol‐disulphide homeostasis |
| url | https://doi.org/10.1002/vms3.70023 |
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