Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis
The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/1162938 |
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| author | Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh |
| author_facet | Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh |
| author_sort | Jayram Karmacharya |
| collection | DOAJ |
| description | The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. Using comparative genomics, we showed that PMAC25284 has copper-transporting ATPases, copper chaperone, copper-responsive transcriptional regulator, and multi-copper oxidase domains, which are found in both Gram-positive (like Mycobacterium tuberculosis and Enterococcus hirae) and Gram-negative bacteria (like E. coli and Pseudomonas aeruginosa). The existence of 4 multi-copper oxidase genes, which supplied an additional copper defense mechanism, could be intriguing information regarding Gram-positive bacteria such as Arthrobacter sp. PAMC25284. In addition, our strain PAMC25284 has the same MmcO gene as M. tuberculosis, with a locus tag KY499_RS04055 similarity of 40.61%, which is the highest among the Gram-positive and Gram-negative bacteria studied for this gene. Our cold-adapted Arthrobacter sp. strain PAMC25564 was published previously but did not contain a multi-copper oxidase domain-containing gene, but strain PAMC25284 was studied in this study. |
| format | Article |
| id | doaj-art-7f9cc35f56214c8fba9da79ed3dca1a9 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-7f9cc35f56214c8fba9da79ed3dca1a92025-02-03T01:24:10ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/1162938Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic AnalysisJayram Karmacharya0Prasansah Shrestha1So-Ra Han2Hyun Park3Tae-Jin Oh4Department of Life Science and Biochemical EngineeringDepartment of Life Science and Biochemical EngineeringDepartment of Life Science and Biochemical EngineeringDivision of BiotechnologyDepartment of Life Science and Biochemical EngineeringThe genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. Using comparative genomics, we showed that PMAC25284 has copper-transporting ATPases, copper chaperone, copper-responsive transcriptional regulator, and multi-copper oxidase domains, which are found in both Gram-positive (like Mycobacterium tuberculosis and Enterococcus hirae) and Gram-negative bacteria (like E. coli and Pseudomonas aeruginosa). The existence of 4 multi-copper oxidase genes, which supplied an additional copper defense mechanism, could be intriguing information regarding Gram-positive bacteria such as Arthrobacter sp. PAMC25284. In addition, our strain PAMC25284 has the same MmcO gene as M. tuberculosis, with a locus tag KY499_RS04055 similarity of 40.61%, which is the highest among the Gram-positive and Gram-negative bacteria studied for this gene. Our cold-adapted Arthrobacter sp. strain PAMC25564 was published previously but did not contain a multi-copper oxidase domain-containing gene, but strain PAMC25284 was studied in this study.http://dx.doi.org/10.1155/2022/1162938 |
| spellingShingle | Jayram Karmacharya Prasansah Shrestha So-Ra Han Hyun Park Tae-Jin Oh Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis International Journal of Microbiology |
| title | Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
| title_full | Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
| title_fullStr | Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
| title_full_unstemmed | Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
| title_short | Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis |
| title_sort | complete genome sequencing of polar arthrobacter sp pamc25284 copper tolerance potential unraveled with genomic analysis |
| url | http://dx.doi.org/10.1155/2022/1162938 |
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