An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium
An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 day...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-02-01
|
| Series: | Mycobiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2025.2452640 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540469833236480 |
|---|---|
| author | Yu Jin Kim Seung Hyo Baek Elena Volynchikova Mee Kyung Sang Ki Deok Kim |
| author_facet | Yu Jin Kim Seung Hyo Baek Elena Volynchikova Mee Kyung Sang Ki Deok Kim |
| author_sort | Yu Jin Kim |
| collection | DOAJ |
| description | An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virulence tests of isolate HSv05 revealed that high concentrations of zoospores (5 × 102 − 105 zoospores/ml) induced severe rot symptoms; however, a low concentration of 102 zoospores/ml produced only considerably weak symptoms on inoculated potato tubers. No symptoms appeared in tubers inoculated with 0 (uninoculated control) or 101 zoospores/ml. These findings suggest that our sporulation method can help obtain adequate zoospores for various basic and applied studies, including pathogen identification, virulence assessment, resistance resource screening, and control strategy development. |
| format | Article |
| id | doaj-art-7f98dea6d4484984a4b5afd59f6796e5 |
| institution | Kabale University |
| issn | 1229-8093 2092-9323 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj-art-7f98dea6d4484984a4b5afd59f6796e52025-02-05T04:09:10ZengTaylor & Francis GroupMycobiology1229-80932092-93232025-02-011810.1080/12298093.2025.2452640An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture MediumYu Jin Kim0Seung Hyo Baek1Elena Volynchikova2Mee Kyung Sang3Ki Deok Kim4Laboratory of Plant Disease and Biocontrol, Department of Plant Biotechnology, Korea University, Seoul, KoreaLaboratory of Plant Disease and Biocontrol, Department of Plant Biotechnology, Korea University, Seoul, KoreaLaboratory of Plant Disease and Biocontrol, Department of Plant Biotechnology, Korea University, Seoul, KoreaDivision of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, KoreaLaboratory of Plant Disease and Biocontrol, Department of Plant Biotechnology, Korea University, Seoul, KoreaAn improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virulence tests of isolate HSv05 revealed that high concentrations of zoospores (5 × 102 − 105 zoospores/ml) induced severe rot symptoms; however, a low concentration of 102 zoospores/ml produced only considerably weak symptoms on inoculated potato tubers. No symptoms appeared in tubers inoculated with 0 (uninoculated control) or 101 zoospores/ml. These findings suggest that our sporulation method can help obtain adequate zoospores for various basic and applied studies, including pathogen identification, virulence assessment, resistance resource screening, and control strategy development.https://www.tandfonline.com/doi/10.1080/12298093.2025.2452640Oomycetespotato leakPythium species. Pythium aphanidermatumsporulation methodzoospore |
| spellingShingle | Yu Jin Kim Seung Hyo Baek Elena Volynchikova Mee Kyung Sang Ki Deok Kim An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium Mycobiology Oomycetes potato leak Pythium species. Pythium aphanidermatum sporulation method zoospore |
| title | An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium |
| title_full | An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium |
| title_fullStr | An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium |
| title_full_unstemmed | An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium |
| title_short | An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium |
| title_sort | improved sporulation method for producing asexual zoospores of pythium aphanidermatum on culture medium |
| topic | Oomycetes potato leak Pythium species. Pythium aphanidermatum sporulation method zoospore |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2025.2452640 |
| work_keys_str_mv | AT yujinkim animprovedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT seunghyobaek animprovedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT elenavolynchikova animprovedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT meekyungsang animprovedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT kideokkim animprovedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT yujinkim improvedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT seunghyobaek improvedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT elenavolynchikova improvedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT meekyungsang improvedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium AT kideokkim improvedsporulationmethodforproducingasexualzoosporesofpythiumaphanidermatumonculturemedium |