Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members
Keratoconus is a progressive corneal ectasia that may lead to severe visual impairment due to the irregular astigmatism caused by corneal thinning. In addition to its association with atopy, eye rubbing, or genetic component, late reports suggest the involvement of inflammation in the pathogenesis o...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/4285268 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563787284086784 |
|---|---|
| author | Ioana Catalina Ionescu Catalina Gabriela Corbu Cristiana Tanase Gabriela Ionita Cristina Nicula Valeria Coviltir Vasile Potop Mihaela Constantin Elena Codrici Simona Mihai Ionela Daniela Popescu Ana-Maria Enciu Dana Dascalescu Miruna Burcel Radu Ciuluvica Liliana-Mary Voinea |
| author_facet | Ioana Catalina Ionescu Catalina Gabriela Corbu Cristiana Tanase Gabriela Ionita Cristina Nicula Valeria Coviltir Vasile Potop Mihaela Constantin Elena Codrici Simona Mihai Ionela Daniela Popescu Ana-Maria Enciu Dana Dascalescu Miruna Burcel Radu Ciuluvica Liliana-Mary Voinea |
| author_sort | Ioana Catalina Ionescu |
| collection | DOAJ |
| description | Keratoconus is a progressive corneal ectasia that may lead to severe visual impairment due to the irregular astigmatism caused by corneal thinning. In addition to its association with atopy, eye rubbing, or genetic component, late reports suggest the involvement of inflammation in the pathogenesis of the disease. Our aim was to determine the concentration of IL-4, IL-6, IL-10, RANTES, IFN gamma, and TNF alpha in the tear film of patients with keratoconus and their first degree family members. We analyzed forty-eight participants in an observational cross-sectional study. The diagnosis of keratoconus had to be confirmed in addition to a minimum of 47 D corneal refractive power by corneal topography readings provided by a Placido-based topography system and analysis of the pattern: irregular astigmatism with an asymmetric “bow-tie.” As for the other groups, the most important diagnosis criteria were a normal topographic pattern with a regular astigmatism. 17 keratoconus patients, 16 relatives, and 15 controls were recruited after clinical assessment as part of the research. The cytokine’s mean values were similar in the keratoconus group and the relatives’ samples but significantly higher compared to the controls. Important differences were found in IL-4 levels between keratoconus patients and relatives and between relatives and controls (mean difference of 302.42, p<0.0016 and 219.16, p<0.033, Tukey’s HSD procedure). In the keratoconus group, using the CORR procedure, we found statistically strong correlations of IL-6 lacrimal concentrations with the disease stage (r=0.56, p<0.01), keratometry (r=0.55, p<0.02), pachymetry (r=−0.64, p<0.048), and corneal hysteresis (r=−0.53, p<0.02). Cytokine overexpression may be relevant for the inflammatory etiology of keratoconus. In conclusion, in the case of some first degree family members, the elevated tear biomarkers may represent a supplementary risk factor. |
| format | Article |
| id | doaj-art-7f8ca4401551499fa6471a13e864f1d6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7f8ca4401551499fa6471a13e864f1d62025-02-03T01:12:32ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/42852684285268Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family MembersIoana Catalina Ionescu0Catalina Gabriela Corbu1Cristiana Tanase2Gabriela Ionita3Cristina Nicula4Valeria Coviltir5Vasile Potop6Mihaela Constantin7Elena Codrici8Simona Mihai9Ionela Daniela Popescu10Ana-Maria Enciu11Dana Dascalescu12Miruna Burcel13Radu Ciuluvica14Liliana-Mary Voinea15Clinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaClinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaBiochemistry-Proteomics Department, Victor Babes National Institute of Pathology, Splaiul Independentei 99-101, Sector 5, 050096 Bucharest, RomaniaInstitute of Chemistry Physics “Ilie Murgulescu”, 202 Splaiul Independentei, 060021 Bucharest, RomaniaDivision of Ophthalmology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaClinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaClinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaOftaclinic Ophthalmology Clinic, Bd. Marasesti 2B, Bucharest, RomaniaBiochemistry-Proteomics Department, Victor Babes National Institute of Pathology, Splaiul Independentei 99-101, Sector 5, 050096 Bucharest, RomaniaBiochemistry-Proteomics Department, Victor Babes National Institute of Pathology, Splaiul Independentei 99-101, Sector 5, 050096 Bucharest, RomaniaBiochemistry-Proteomics Department, Victor Babes National Institute of Pathology, Splaiul Independentei 99-101, Sector 5, 050096 Bucharest, RomaniaBiochemistry-Proteomics Department, Victor Babes National Institute of Pathology, Splaiul Independentei 99-101, Sector 5, 050096 Bucharest, RomaniaClinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaClinical Hospital of Ophthalmologic Emergencies, Alexandru Lahovari 1 Square, Bucharest, RomaniaFaculty of Dental Medicine, University of Medicine and Pharmacy “Carol Davila”, Bulevardul Eroii Sanitari 8, 050474 Bucharest, RomaniaDivision of Ophthalmology, Faculty of Medicine, University of Medicine and Pharmacy “Carol Davila”, Bulevardul Eroii Sanitari 8, Sector 5, 050474 Bucharest, RomaniaKeratoconus is a progressive corneal ectasia that may lead to severe visual impairment due to the irregular astigmatism caused by corneal thinning. In addition to its association with atopy, eye rubbing, or genetic component, late reports suggest the involvement of inflammation in the pathogenesis of the disease. Our aim was to determine the concentration of IL-4, IL-6, IL-10, RANTES, IFN gamma, and TNF alpha in the tear film of patients with keratoconus and their first degree family members. We analyzed forty-eight participants in an observational cross-sectional study. The diagnosis of keratoconus had to be confirmed in addition to a minimum of 47 D corneal refractive power by corneal topography readings provided by a Placido-based topography system and analysis of the pattern: irregular astigmatism with an asymmetric “bow-tie.” As for the other groups, the most important diagnosis criteria were a normal topographic pattern with a regular astigmatism. 17 keratoconus patients, 16 relatives, and 15 controls were recruited after clinical assessment as part of the research. The cytokine’s mean values were similar in the keratoconus group and the relatives’ samples but significantly higher compared to the controls. Important differences were found in IL-4 levels between keratoconus patients and relatives and between relatives and controls (mean difference of 302.42, p<0.0016 and 219.16, p<0.033, Tukey’s HSD procedure). In the keratoconus group, using the CORR procedure, we found statistically strong correlations of IL-6 lacrimal concentrations with the disease stage (r=0.56, p<0.01), keratometry (r=0.55, p<0.02), pachymetry (r=−0.64, p<0.048), and corneal hysteresis (r=−0.53, p<0.02). Cytokine overexpression may be relevant for the inflammatory etiology of keratoconus. In conclusion, in the case of some first degree family members, the elevated tear biomarkers may represent a supplementary risk factor.http://dx.doi.org/10.1155/2018/4285268 |
| spellingShingle | Ioana Catalina Ionescu Catalina Gabriela Corbu Cristiana Tanase Gabriela Ionita Cristina Nicula Valeria Coviltir Vasile Potop Mihaela Constantin Elena Codrici Simona Mihai Ionela Daniela Popescu Ana-Maria Enciu Dana Dascalescu Miruna Burcel Radu Ciuluvica Liliana-Mary Voinea Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members Mediators of Inflammation |
| title | Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members |
| title_full | Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members |
| title_fullStr | Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members |
| title_full_unstemmed | Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members |
| title_short | Overexpression of Tear Inflammatory Cytokines as Additional Finding in Keratoconus Patients and Their First Degree Family Members |
| title_sort | overexpression of tear inflammatory cytokines as additional finding in keratoconus patients and their first degree family members |
| url | http://dx.doi.org/10.1155/2018/4285268 |
| work_keys_str_mv | AT ioanacatalinaionescu overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT catalinagabrielacorbu overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT cristianatanase overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT gabrielaionita overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT cristinanicula overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT valeriacoviltir overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT vasilepotop overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT mihaelaconstantin overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT elenacodrici overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT simonamihai overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT ioneladanielapopescu overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT anamariaenciu overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT danadascalescu overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT mirunaburcel overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT raduciuluvica overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers AT lilianamaryvoinea overexpressionoftearinflammatorycytokinesasadditionalfindinginkeratoconuspatientsandtheirfirstdegreefamilymembers |