Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells
The main aim of the current research was to study the effect of porphyra-334, one of mycosporine-like amino acids (MAAs), well known as UV-absorbing compounds, on UVinduced apoptosis in human immortalized keratinocyte (HaCaT) cells. Due to their UV-screening capacity and ability to prevent UV-induce...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2017-06-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/acph-2017-0015 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570273082114048 |
|---|---|
| author | Suh Sung-Suk Oh Se Kyung Lee Sung Gu Kim Il-Chan Kim Sanghee |
| author_facet | Suh Sung-Suk Oh Se Kyung Lee Sung Gu Kim Il-Chan Kim Sanghee |
| author_sort | Suh Sung-Suk |
| collection | DOAJ |
| description | The main aim of the current research was to study the effect of porphyra-334, one of mycosporine-like amino acids (MAAs), well known as UV-absorbing compounds, on UVinduced apoptosis in human immortalized keratinocyte (HaCaT) cells. Due to their UV-screening capacity and ability to prevent UV-induced DNA damage, MAAs have recently attracted considerable attention in both industry and research in pharmacology. Herein, human HaCaT cells were used to determine the biological activities of porphyra- 334 by various in vitro assays, including proliferation, apoptosis and Western blot assays. The proliferation rate of UV-irradiated HaCaT cells was significantly decreased compared to the control group. Pretreatment with porphyra- 334 markedly attenuated the inhibitory effect of UV and induced a dramatic decrease in the apoptotic rate. Expression of active caspase-3 protein was increased in response to UV irradiation, while caspase-3 levels were similar between cells treated with porphyra-334 and the non-irradiated control group. Taken together, our data suggest that porphyra-334 inhibits UV-induced apoptosis in HaCaT cells through attenuation of the caspase pathway. |
| format | Article |
| id | doaj-art-7f6685cc009d406f8591f47ab2e14664 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-7f6685cc009d406f8591f47ab2e146642025-02-02T15:49:15ZengSciendoActa Pharmaceutica1846-95582017-06-0167225726410.1515/acph-2017-0015acph-2017-0015Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cellsSuh Sung-Suk0Oh Se Kyung1Lee Sung Gu2Kim Il-Chan3Kim Sanghee4Division of Polar Life Sciences Korea Polar Research Institute, Incheon, 21990, Korea (Republic of)Division of Polar Life Sciences Korea Polar Research Institute, Incheon, 21990, Korea (Republic of)Division of Polar Life Sciences Korea Polar Research Institute Incheon, 21990 Incheon, Korea (Republic of)Division of Polar Life Sciences Korea Polar Research Institute Incheon, 21990 Incheon, Korea (Republic of)Division of Polar Life Sciences Korea Polar Research Institute Incheon, 21990 Incheon, Korea (Republic of)The main aim of the current research was to study the effect of porphyra-334, one of mycosporine-like amino acids (MAAs), well known as UV-absorbing compounds, on UVinduced apoptosis in human immortalized keratinocyte (HaCaT) cells. Due to their UV-screening capacity and ability to prevent UV-induced DNA damage, MAAs have recently attracted considerable attention in both industry and research in pharmacology. Herein, human HaCaT cells were used to determine the biological activities of porphyra- 334 by various in vitro assays, including proliferation, apoptosis and Western blot assays. The proliferation rate of UV-irradiated HaCaT cells was significantly decreased compared to the control group. Pretreatment with porphyra- 334 markedly attenuated the inhibitory effect of UV and induced a dramatic decrease in the apoptotic rate. Expression of active caspase-3 protein was increased in response to UV irradiation, while caspase-3 levels were similar between cells treated with porphyra-334 and the non-irradiated control group. Taken together, our data suggest that porphyra-334 inhibits UV-induced apoptosis in HaCaT cells through attenuation of the caspase pathway.https://doi.org/10.1515/acph-2017-0015porphyra-334apoptosiscell proliferationuv irradiationhacat cells |
| spellingShingle | Suh Sung-Suk Oh Se Kyung Lee Sung Gu Kim Il-Chan Kim Sanghee Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells Acta Pharmaceutica porphyra-334 apoptosis cell proliferation uv irradiation hacat cells |
| title | Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells |
| title_full | Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells |
| title_fullStr | Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells |
| title_full_unstemmed | Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells |
| title_short | Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells |
| title_sort | porphyra 334 a mycosporine like amino acid attenuates uv induced apoptosis in hacat cells |
| topic | porphyra-334 apoptosis cell proliferation uv irradiation hacat cells |
| url | https://doi.org/10.1515/acph-2017-0015 |
| work_keys_str_mv | AT suhsungsuk porphyra334amycosporinelikeaminoacidattenuatesuvinducedapoptosisinhacatcells AT ohsekyung porphyra334amycosporinelikeaminoacidattenuatesuvinducedapoptosisinhacatcells AT leesunggu porphyra334amycosporinelikeaminoacidattenuatesuvinducedapoptosisinhacatcells AT kimilchan porphyra334amycosporinelikeaminoacidattenuatesuvinducedapoptosisinhacatcells AT kimsanghee porphyra334amycosporinelikeaminoacidattenuatesuvinducedapoptosisinhacatcells |