Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep
SARS-CoV-2 can cause clinical and inapparent disease and mortality in several animals cohabitating with humans, and sheep are susceptible to SARS-CoV-2 due to virus–receptor interactions similar to those in humans. Hence, sheep have the potential to be infected, spread, and develop neutralising anti...
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MDPI AG
2024-12-01
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| Series: | Microorganisms |
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| author | Milena Samojlović João R. Mesquita Sérgio Santos-Silva Malin Neptin Joakim Esbjörnsson |
| author_facet | Milena Samojlović João R. Mesquita Sérgio Santos-Silva Malin Neptin Joakim Esbjörnsson |
| author_sort | Milena Samojlović |
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| description | SARS-CoV-2 can cause clinical and inapparent disease and mortality in several animals cohabitating with humans, and sheep are susceptible to SARS-CoV-2 due to virus–receptor interactions similar to those in humans. Hence, sheep have the potential to be infected, spread, and develop neutralising antibodies (NAbs) against SARS-CoV-2. The aim of this study was to investigate the prevalence of SARS-CoV-2 NAbs in farm animals after natural exposure to the virus. Serum samples were collected from sheep in the Serra da Estrela region in Portugal, both prior to and during the COVID-19 pandemic. The sera were tested by established SARS-CoV-2 pseudovirus systems for multiple SARS-CoV-2 variants (early—Wuhan, mid—Delta, Omicron—BA.1, and late—Omicron XBB, BQ.1.1). Partial neutralisation activity in Pre-pandemic and Mid-pandemic samples was observed, while no NAb activity was observed in Late-pandemic samples tested. Different levels of NAbs were observed between Pre-pandemic samples and those collected during the Mid-pandemic and Late-pandemic periods (<i>p</i> ≤ 0.01). Our results indicate that SARS-CoV-2 cross-species transmission may have occurred through human–sheep contacts on sheep farms during the pandemic, and that farm animals could contribute to the One Health Approach in zoonotic virus surveillance and pandemic preparedness. |
| format | Article |
| id | doaj-art-7f43e3ed4a2b498eb19f408831ea5bef |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microorganisms |
| spelling | doaj-art-7f43e3ed4a2b498eb19f408831ea5bef2025-01-24T13:42:25ZengMDPI AGMicroorganisms2076-26072024-12-011314910.3390/microorganisms13010049Investigating SARS-CoV-2 Neutralising Antibody Response in SheepMilena Samojlović0João R. Mesquita1Sérgio Santos-Silva2Malin Neptin3Joakim Esbjörnsson4Systems Virology, Faculty of Medicine, Lund University, 223 62 Lund, SwedenInstituto de Ciências Biomédicas Abel Salazar, University of Porto, 4050-313 Porto, PortugalInstituto de Ciências Biomédicas Abel Salazar, University of Porto, 4050-313 Porto, PortugalSystems Virology, Faculty of Medicine, Lund University, 223 62 Lund, SwedenSystems Virology, Faculty of Medicine, Lund University, 223 62 Lund, SwedenSARS-CoV-2 can cause clinical and inapparent disease and mortality in several animals cohabitating with humans, and sheep are susceptible to SARS-CoV-2 due to virus–receptor interactions similar to those in humans. Hence, sheep have the potential to be infected, spread, and develop neutralising antibodies (NAbs) against SARS-CoV-2. The aim of this study was to investigate the prevalence of SARS-CoV-2 NAbs in farm animals after natural exposure to the virus. Serum samples were collected from sheep in the Serra da Estrela region in Portugal, both prior to and during the COVID-19 pandemic. The sera were tested by established SARS-CoV-2 pseudovirus systems for multiple SARS-CoV-2 variants (early—Wuhan, mid—Delta, Omicron—BA.1, and late—Omicron XBB, BQ.1.1). Partial neutralisation activity in Pre-pandemic and Mid-pandemic samples was observed, while no NAb activity was observed in Late-pandemic samples tested. Different levels of NAbs were observed between Pre-pandemic samples and those collected during the Mid-pandemic and Late-pandemic periods (<i>p</i> ≤ 0.01). Our results indicate that SARS-CoV-2 cross-species transmission may have occurred through human–sheep contacts on sheep farms during the pandemic, and that farm animals could contribute to the One Health Approach in zoonotic virus surveillance and pandemic preparedness.https://www.mdpi.com/2076-2607/13/1/49one health approachSARS-CoV-2sheepsentinelspandemic preparedness |
| spellingShingle | Milena Samojlović João R. Mesquita Sérgio Santos-Silva Malin Neptin Joakim Esbjörnsson Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep Microorganisms one health approach SARS-CoV-2 sheep sentinels pandemic preparedness |
| title | Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep |
| title_full | Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep |
| title_fullStr | Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep |
| title_full_unstemmed | Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep |
| title_short | Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep |
| title_sort | investigating sars cov 2 neutralising antibody response in sheep |
| topic | one health approach SARS-CoV-2 sheep sentinels pandemic preparedness |
| url | https://www.mdpi.com/2076-2607/13/1/49 |
| work_keys_str_mv | AT milenasamojlovic investigatingsarscov2neutralisingantibodyresponseinsheep AT joaormesquita investigatingsarscov2neutralisingantibodyresponseinsheep AT sergiosantossilva investigatingsarscov2neutralisingantibodyresponseinsheep AT malinneptin investigatingsarscov2neutralisingantibodyresponseinsheep AT joakimesbjornsson investigatingsarscov2neutralisingantibodyresponseinsheep |