Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy
Despite increased use of monoclonal and polyclonal antibody therapies, including during pregnancy, there is little data on appropriate animal models that could humanely be used to understand determinants of protection and to evaluate safety of these biologics in the mother and the developing fetus....
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/538701 |
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| author | Evi Budo Struble Li Ma Lilin Zhong A. Lesher Joel Beren Pei Zhang |
| author_facet | Evi Budo Struble Li Ma Lilin Zhong A. Lesher Joel Beren Pei Zhang |
| author_sort | Evi Budo Struble |
| collection | DOAJ |
| description | Despite increased use of monoclonal and polyclonal antibody therapies, including during pregnancy, there is little data on appropriate animal models that could humanely be used to understand determinants of protection and to evaluate safety of these biologics in the mother and the developing fetus. Here, we demonstrate that pregnant guinea pigs can transport human IgG transplacentally at the end of pregnancy. We also observe that human IgG binds to an engineered soluble variant of the guinea pig neonatal Fc receptor in vitro in a manner similar to that demonstrated for the human variant, suggesting that this transplacental transport mirrors the receptor-based mechanism seen in humans. Using an intravenous antihepatitis B-specific immune globulin preparation as an example, we show that this transport results in neutralizing activity in the mother and the newborn that would potentially be prophylactic against hepatitis B viral infection. These preliminary data lay the groundwork for introducing pregnant guinea pigs as an appropriate model for the evaluation of antibody therapies and advancing the health of women and neonates. |
| format | Article |
| id | doaj-art-7f13f059236f4075901b34704da2d58a |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-7f13f059236f4075901b34704da2d58a2025-02-03T06:11:00ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/538701538701Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during PregnancyEvi Budo Struble0Li Ma1Lilin Zhong2A. Lesher3Joel Beren4Pei Zhang5Laboratory of Plasma Derivatives, Division of Hematology, OBRR, FDA, Bethesda, MD 20892, USALaboratory of Plasma Derivatives, Division of Hematology, OBRR, FDA, Bethesda, MD 20892, USALaboratory of Plasma Derivatives, Division of Hematology, OBRR, FDA, Bethesda, MD 20892, USADivision of Veterinary Services, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USADivision of Veterinary Services, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USALaboratory of Plasma Derivatives, Division of Hematology, OBRR, FDA, Bethesda, MD 20892, USADespite increased use of monoclonal and polyclonal antibody therapies, including during pregnancy, there is little data on appropriate animal models that could humanely be used to understand determinants of protection and to evaluate safety of these biologics in the mother and the developing fetus. Here, we demonstrate that pregnant guinea pigs can transport human IgG transplacentally at the end of pregnancy. We also observe that human IgG binds to an engineered soluble variant of the guinea pig neonatal Fc receptor in vitro in a manner similar to that demonstrated for the human variant, suggesting that this transplacental transport mirrors the receptor-based mechanism seen in humans. Using an intravenous antihepatitis B-specific immune globulin preparation as an example, we show that this transport results in neutralizing activity in the mother and the newborn that would potentially be prophylactic against hepatitis B viral infection. These preliminary data lay the groundwork for introducing pregnant guinea pigs as an appropriate model for the evaluation of antibody therapies and advancing the health of women and neonates.http://dx.doi.org/10.1155/2012/538701 |
| spellingShingle | Evi Budo Struble Li Ma Lilin Zhong A. Lesher Joel Beren Pei Zhang Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy Clinical and Developmental Immunology |
| title | Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy |
| title_full | Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy |
| title_fullStr | Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy |
| title_full_unstemmed | Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy |
| title_short | Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy |
| title_sort | human antibodies can cross guinea pig placenta and bind its neonatal fc receptor implications for studying immune prophylaxis and therapy during pregnancy |
| url | http://dx.doi.org/10.1155/2012/538701 |
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