Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae
In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial e...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/8903982 |
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| author | Christie F. Michael Christopher M. Waters Kim S. LeMessurier Amali E. Samarasinghe Chi Y. Song Kafait U. Malik D. Betty Lew |
| author_facet | Christie F. Michael Christopher M. Waters Kim S. LeMessurier Amali E. Samarasinghe Chi Y. Song Kafait U. Malik D. Betty Lew |
| author_sort | Christie F. Michael |
| collection | DOAJ |
| description | In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors. |
| format | Article |
| id | doaj-art-7f13b9a817454e1cad8cdb5ae5e65d0c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7f13b9a817454e1cad8cdb5ae5e65d0c2025-02-03T01:01:37ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/89039828903982Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiaeChristie F. Michael0Christopher M. Waters1Kim S. LeMessurier2Amali E. Samarasinghe3Chi Y. Song4Kafait U. Malik5D. Betty Lew6Department of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38103, USADepartment of Physiology, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38163, USADepartment of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38103, USADepartment of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38103, USADepartment of Pharmacology, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38163, USADepartment of Pharmacology, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38163, USADepartment of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38103, USAIn asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.http://dx.doi.org/10.1155/2017/8903982 |
| spellingShingle | Christie F. Michael Christopher M. Waters Kim S. LeMessurier Amali E. Samarasinghe Chi Y. Song Kafait U. Malik D. Betty Lew Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae Journal of Immunology Research |
| title | Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae |
| title_full | Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae |
| title_fullStr | Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae |
| title_full_unstemmed | Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae |
| title_short | Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae |
| title_sort | airway epithelial repair by a prebiotic mannan derived from saccharomyces cerevisiae |
| url | http://dx.doi.org/10.1155/2017/8903982 |
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