Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study
Radix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries. Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may b...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/4508374 |
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| author | Wenhao Cheng Yinghui Li Wei Yang Siyang Wu Mengmeng Wei Yang Gao Chen Kang Shuofeng Zhang Yingfei Li |
| author_facet | Wenhao Cheng Yinghui Li Wei Yang Siyang Wu Mengmeng Wei Yang Gao Chen Kang Shuofeng Zhang Yingfei Li |
| author_sort | Wenhao Cheng |
| collection | DOAJ |
| description | Radix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries. Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may be related to these constituents. However, information on the pharmacokinetics of stilbenes and anthraquinones at a subtoxic dose of RPM is limited. A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established. Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects. Chromatographic separation was performed using a waters HSS C18 column with a gradient elution using water and acetonitrile both containing 0.025% formic acid within a run time of 9 min. The constituents were detected in negative ionization mode using multiple reaction monitoring. The method was fully validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects, and stability. The lower limit of quantitation of the analytes was 0.1–1 ng/mL. The intrabatch and interbatch accuracies were 87.1–109%, and the precision was within the acceptable limits. The method was applied to a pharmacokinetic study after oral administration of RPM extract to rats at a subtoxic dose of 36 g/kg. |
| format | Article |
| id | doaj-art-7f0c5c45a2d8458aaed0c19802193d42 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-7f0c5c45a2d8458aaed0c19802193d422025-02-03T06:46:36ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/45083744508374Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic StudyWenhao Cheng0Yinghui Li1Wei Yang2Siyang Wu3Mengmeng Wei4Yang Gao5Chen Kang6Shuofeng Zhang7Yingfei Li8School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaCenter for DMPK Research of Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese, Beijing 100700, ChinaRadix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries. Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may be related to these constituents. However, information on the pharmacokinetics of stilbenes and anthraquinones at a subtoxic dose of RPM is limited. A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established. Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects. Chromatographic separation was performed using a waters HSS C18 column with a gradient elution using water and acetonitrile both containing 0.025% formic acid within a run time of 9 min. The constituents were detected in negative ionization mode using multiple reaction monitoring. The method was fully validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects, and stability. The lower limit of quantitation of the analytes was 0.1–1 ng/mL. The intrabatch and interbatch accuracies were 87.1–109%, and the precision was within the acceptable limits. The method was applied to a pharmacokinetic study after oral administration of RPM extract to rats at a subtoxic dose of 36 g/kg.http://dx.doi.org/10.1155/2020/4508374 |
| spellingShingle | Wenhao Cheng Yinghui Li Wei Yang Siyang Wu Mengmeng Wei Yang Gao Chen Kang Shuofeng Zhang Yingfei Li Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study International Journal of Analytical Chemistry |
| title | Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study |
| title_full | Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study |
| title_fullStr | Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study |
| title_full_unstemmed | Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study |
| title_short | Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study |
| title_sort | simultaneous determination of 13 constituents of radix polygoni multiflori in rat plasma and its application in a pharmacokinetic study |
| url | http://dx.doi.org/10.1155/2020/4508374 |
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