Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin
The unique redox properties of nanoscale cerium dioxide determine its diverse application in biology and medicine as a regulator of oxidative metabolism. Lipid modifiers of the nanoparticle surface change their biochemical properties and bioavailability. Complexes with lipids can be formed upon cont...
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| Format: | Article |
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2025-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/1/53 |
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| author | Elena V. Proskurnina Madina M. Sozarukova Elizaveta S. Ershova Ekaterina A. Savinova Larisa V. Kameneva Natalia N. Veiko Maria A. Teplonogova Vladimir P. Saprykin Vladimir K. Ivanov Svetlana V. Kostyuk |
| author_facet | Elena V. Proskurnina Madina M. Sozarukova Elizaveta S. Ershova Ekaterina A. Savinova Larisa V. Kameneva Natalia N. Veiko Maria A. Teplonogova Vladimir P. Saprykin Vladimir K. Ivanov Svetlana V. Kostyuk |
| author_sort | Elena V. Proskurnina |
| collection | DOAJ |
| description | The unique redox properties of nanoscale cerium dioxide determine its diverse application in biology and medicine as a regulator of oxidative metabolism. Lipid modifiers of the nanoparticle surface change their biochemical properties and bioavailability. Complexes with lipids can be formed upon contact of the nanoparticles with the membrane. The effects of lipid coating on nanoceria have not been studied yet. Here, we assessed the effect of bare and cardiolipin-coated CeO<sub>2</sub> on the expression of oxidative metabolism genes in human embryonic lung fibroblasts. Cell viability, mitochondrial activity, intracellular reactive oxygen species, NOX4, NRF2, and NF-κB expression, oxidative DNA damage/repair, autophagy, and cell proliferation were studied. We used an MTT assay, fluorescence microscopy, real-time reverse transcription polymerase chain reaction, and flow cytometry. At a concentration of 1.5 μM, bare and cardiolipin-coated nanoceria penetrated into cells within 1–3 h. Cell survival, mitochondrial activity, and the proliferative effect were similar for bare and cardiolipin-coated nanoceria. Intracellular ROS, activation of NOX4, NRF2, and NF-kB, DNA oxidative damage, and DNA break/repair were different. Cardiolipin-coated nanoceria induced intracellular oxidative stress and short-term activation of these genes and DNA damage/break/repair. Unlike bare nanoceria, cardiolipin-coated nanoceria induced autophagy. Thus, the effects of cardiolipin-coated nanoceria are determined by both the nanoceria itself and cardiolipin. Presumably, the differences in properties are due to lipid peroxidation of cardiolipin. This effect needs to be taken into account when developing nanoceria-based drugs targeting mitochondria. |
| format | Article |
| id | doaj-art-7f05191c18a548eb8f6274738da476e3 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-7f05191c18a548eb8f6274738da476e32025-01-24T13:25:00ZengMDPI AGBiomolecules2218-273X2025-01-011515310.3390/biom15010053Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of CardiolipinElena V. Proskurnina0Madina M. Sozarukova1Elizaveta S. Ershova2Ekaterina A. Savinova3Larisa V. Kameneva4Natalia N. Veiko5Maria A. Teplonogova6Vladimir P. Saprykin7Vladimir K. Ivanov8Svetlana V. Kostyuk9Research Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Leninskii Prospect 31, Moscow 119071, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Leninskii Prospect 31, Moscow 119071, RussiaFaculty of Biotechnology and Fisheries, K.G. Razumovsky Moscow State University of Technologies and Management, Zemlyanoy Val Str. 73, Moscow 109004, RussiaKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Leninskii Prospect 31, Moscow 119071, RussiaResearch Centre for Medical Genetics, ul. Moskvorechye 1, Moscow 115522, RussiaThe unique redox properties of nanoscale cerium dioxide determine its diverse application in biology and medicine as a regulator of oxidative metabolism. Lipid modifiers of the nanoparticle surface change their biochemical properties and bioavailability. Complexes with lipids can be formed upon contact of the nanoparticles with the membrane. The effects of lipid coating on nanoceria have not been studied yet. Here, we assessed the effect of bare and cardiolipin-coated CeO<sub>2</sub> on the expression of oxidative metabolism genes in human embryonic lung fibroblasts. Cell viability, mitochondrial activity, intracellular reactive oxygen species, NOX4, NRF2, and NF-κB expression, oxidative DNA damage/repair, autophagy, and cell proliferation were studied. We used an MTT assay, fluorescence microscopy, real-time reverse transcription polymerase chain reaction, and flow cytometry. At a concentration of 1.5 μM, bare and cardiolipin-coated nanoceria penetrated into cells within 1–3 h. Cell survival, mitochondrial activity, and the proliferative effect were similar for bare and cardiolipin-coated nanoceria. Intracellular ROS, activation of NOX4, NRF2, and NF-kB, DNA oxidative damage, and DNA break/repair were different. Cardiolipin-coated nanoceria induced intracellular oxidative stress and short-term activation of these genes and DNA damage/break/repair. Unlike bare nanoceria, cardiolipin-coated nanoceria induced autophagy. Thus, the effects of cardiolipin-coated nanoceria are determined by both the nanoceria itself and cardiolipin. Presumably, the differences in properties are due to lipid peroxidation of cardiolipin. This effect needs to be taken into account when developing nanoceria-based drugs targeting mitochondria.https://www.mdpi.com/2218-273X/15/1/53cardiolipin-coated nanoceriaoxidative metabolism genesoxidative DNA damagecell proliferationautophagyhuman embryonic lung fibroblasts |
| spellingShingle | Elena V. Proskurnina Madina M. Sozarukova Elizaveta S. Ershova Ekaterina A. Savinova Larisa V. Kameneva Natalia N. Veiko Maria A. Teplonogova Vladimir P. Saprykin Vladimir K. Ivanov Svetlana V. Kostyuk Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin Biomolecules cardiolipin-coated nanoceria oxidative metabolism genes oxidative DNA damage cell proliferation autophagy human embryonic lung fibroblasts |
| title | Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin |
| title_full | Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin |
| title_fullStr | Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin |
| title_full_unstemmed | Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin |
| title_short | Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin |
| title_sort | lipid coating modulates effects of nanoceria on oxidative metabolism in human embryonic lung fibroblasts a case of cardiolipin |
| topic | cardiolipin-coated nanoceria oxidative metabolism genes oxidative DNA damage cell proliferation autophagy human embryonic lung fibroblasts |
| url | https://www.mdpi.com/2218-273X/15/1/53 |
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