MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation
Abstract Nowadays, the investigation for overcoming tamoxifen (TAM) resistance is confronting a considerable challenge. Therefore, immediate attention is required to elucidate the mechanism underlying TAM resistance in breast cancer. This research primarily aimed to define how miRNA-363-3p facilitat...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-83938-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850048391348748288 |
|---|---|
| author | Yaning Liang Cuiyu Shi Yu Wang Bingjie Fan Wei Song Rong Shen |
| author_facet | Yaning Liang Cuiyu Shi Yu Wang Bingjie Fan Wei Song Rong Shen |
| author_sort | Yaning Liang |
| collection | DOAJ |
| description | Abstract Nowadays, the investigation for overcoming tamoxifen (TAM) resistance is confronting a considerable challenge. Therefore, immediate attention is required to elucidate the mechanism underlying TAM resistance in breast cancer. This research primarily aimed to define how miRNA-363-3p facilitates resistance to TAM in breast cancer. High-throughput miRNA sequencing was performed using RNAs prepared from breast cancer MCF-7 cells and TAM-resistant MCF-7 cells (MCF-7-TAM). An increase in miRNA-363-3p levels was observed in MCF-7-TAM cells. In MCF-7 cells, miRNA-363-3p directly targeted and negatively regulated phosphatase and tensin homolog (PTEN). Reduction of miRNA-363-3p retarded cell growth and accelerated cell apoptosis, thereby enhancing the sensitivity of TAM. Moreover, analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway showed significant enrichment of target genes within the phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. Ultimately, miR-363-3p decreased the responsiveness of breast cancer cells to TAM by targeting and suppressing PTEN through a mechanism associated with the PI3K-Akt pathway. Therefore, these results suggest that miR-363-3p-dependent PTEN expression contributes to the mechanisms underlying breast cancer endocrine resistance. |
| format | Article |
| id | doaj-art-7f027abbd19043e8a03242e5ec3bc9e8 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7f027abbd19043e8a03242e5ec3bc9e82025-08-20T02:53:58ZengNature PortfolioScientific Reports2045-23222024-12-0114111410.1038/s41598-024-83938-8MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulationYaning Liang0Cuiyu Shi1Yu Wang2Bingjie Fan3Wei Song4Rong Shen5Department of Minimally Invasive Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Minimally Invasive Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityTumor Research and Therapy Center, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityCancer Research Center, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical SciencesDepartment of Minimally Invasive Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Minimally Invasive Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityAbstract Nowadays, the investigation for overcoming tamoxifen (TAM) resistance is confronting a considerable challenge. Therefore, immediate attention is required to elucidate the mechanism underlying TAM resistance in breast cancer. This research primarily aimed to define how miRNA-363-3p facilitates resistance to TAM in breast cancer. High-throughput miRNA sequencing was performed using RNAs prepared from breast cancer MCF-7 cells and TAM-resistant MCF-7 cells (MCF-7-TAM). An increase in miRNA-363-3p levels was observed in MCF-7-TAM cells. In MCF-7 cells, miRNA-363-3p directly targeted and negatively regulated phosphatase and tensin homolog (PTEN). Reduction of miRNA-363-3p retarded cell growth and accelerated cell apoptosis, thereby enhancing the sensitivity of TAM. Moreover, analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway showed significant enrichment of target genes within the phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. Ultimately, miR-363-3p decreased the responsiveness of breast cancer cells to TAM by targeting and suppressing PTEN through a mechanism associated with the PI3K-Akt pathway. Therefore, these results suggest that miR-363-3p-dependent PTEN expression contributes to the mechanisms underlying breast cancer endocrine resistance.https://doi.org/10.1038/s41598-024-83938-8Hsa-miR-363-3pBreast cancerPTENTAM resistancePI3K/AKT signaling pathway |
| spellingShingle | Yaning Liang Cuiyu Shi Yu Wang Bingjie Fan Wei Song Rong Shen MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation Scientific Reports Hsa-miR-363-3p Breast cancer PTEN TAM resistance PI3K/AKT signaling pathway |
| title | MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation |
| title_full | MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation |
| title_fullStr | MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation |
| title_full_unstemmed | MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation |
| title_short | MiR-363-3p induces tamoxifen resistance in breast cancer cells through PTEN modulation |
| title_sort | mir 363 3p induces tamoxifen resistance in breast cancer cells through pten modulation |
| topic | Hsa-miR-363-3p Breast cancer PTEN TAM resistance PI3K/AKT signaling pathway |
| url | https://doi.org/10.1038/s41598-024-83938-8 |
| work_keys_str_mv | AT yaningliang mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation AT cuiyushi mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation AT yuwang mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation AT bingjiefan mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation AT weisong mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation AT rongshen mir3633pinducestamoxifenresistanceinbreastcancercellsthroughptenmodulation |