Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook.
The present study was aimed to evaluate the analgesic and anti-inflammatory properties of Castanospermum australe and to profile phytochemicals by GC-MS. The ethanolic extracts were prepared by successive solvent extraction using Soxhlet apparatus. The analgesic activity was analyzed by hot plate me...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/587807 |
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| author | Thankarajan Sajeesh Thangaraj Parimelazhagan |
| author_facet | Thankarajan Sajeesh Thangaraj Parimelazhagan |
| author_sort | Thankarajan Sajeesh |
| collection | DOAJ |
| description | The present study was aimed to evaluate the analgesic and anti-inflammatory properties of Castanospermum australe and to profile phytochemicals by GC-MS. The ethanolic extracts were prepared by successive solvent extraction using Soxhlet apparatus. The analgesic activity was analyzed by hot plate method and acetic acid-induced writhing test whereas anti-inflammatory study was done by carrageenan induced paw oedema model. The acute toxicity study revealed that ethanol extracts of leaf and bark of C. australe were safe even at a higher dose of 2000 mg/kg whereas ethanol extract of seed was toxic at the same dose. In both hot plate method (5.85 s) and acetic acid-induced writhing test (57%), the leaf ethanol extract exhibited significant analgesic activity (P<0.001) at a dose of 400 mg/kg. The anti-inflammatory activity of leaf extract was exhibited by the reduction in paw linear diameter by 64.76% at 400 mg/kg in carrageenan induced paw oedema. The GC-MS analysis of the ethanol extract of leaf revealed sixteen major compounds of which 1,7-dimethyl-4,10-dioxa-1,7-diazacyclododecane, (+)-N-methylephedrine, and permethylspermine were found to be pharmaceutically and the most important. These findings justify that C. australe can be a valuable natural analgesic and anti-inflammatory source which seemed to provide potential phytotherapeutics against various ailments. |
| format | Article |
| id | doaj-art-7efaa38078f8452198e89f95b158cf77 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7efaa38078f8452198e89f95b158cf772025-02-03T05:48:39ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/587807587807Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook.Thankarajan Sajeesh0Thangaraj Parimelazhagan1Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore,Tamil Nadu 641 046, IndiaBioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore,Tamil Nadu 641 046, IndiaThe present study was aimed to evaluate the analgesic and anti-inflammatory properties of Castanospermum australe and to profile phytochemicals by GC-MS. The ethanolic extracts were prepared by successive solvent extraction using Soxhlet apparatus. The analgesic activity was analyzed by hot plate method and acetic acid-induced writhing test whereas anti-inflammatory study was done by carrageenan induced paw oedema model. The acute toxicity study revealed that ethanol extracts of leaf and bark of C. australe were safe even at a higher dose of 2000 mg/kg whereas ethanol extract of seed was toxic at the same dose. In both hot plate method (5.85 s) and acetic acid-induced writhing test (57%), the leaf ethanol extract exhibited significant analgesic activity (P<0.001) at a dose of 400 mg/kg. The anti-inflammatory activity of leaf extract was exhibited by the reduction in paw linear diameter by 64.76% at 400 mg/kg in carrageenan induced paw oedema. The GC-MS analysis of the ethanol extract of leaf revealed sixteen major compounds of which 1,7-dimethyl-4,10-dioxa-1,7-diazacyclododecane, (+)-N-methylephedrine, and permethylspermine were found to be pharmaceutically and the most important. These findings justify that C. australe can be a valuable natural analgesic and anti-inflammatory source which seemed to provide potential phytotherapeutics against various ailments.http://dx.doi.org/10.1155/2014/587807 |
| spellingShingle | Thankarajan Sajeesh Thangaraj Parimelazhagan Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. The Scientific World Journal |
| title | Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. |
| title_full | Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. |
| title_fullStr | Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. |
| title_full_unstemmed | Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. |
| title_short | Analgesic, Anti-Inflammatory, and GC-MS Studies on Castanospermum australe A. Cunn. & C. Fraser ex Hook. |
| title_sort | analgesic anti inflammatory and gc ms studies on castanospermum australe a cunn c fraser ex hook |
| url | http://dx.doi.org/10.1155/2014/587807 |
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