Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen
The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and b...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2013/165348 |
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| author | P. Perumal Kezhavituo Vupru C. Rajkhowa |
| author_facet | P. Perumal Kezhavituo Vupru C. Rajkhowa |
| author_sort | P. Perumal |
| collection | DOAJ |
| description | The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliquots and diluted with the TEYC extender. Group 1: semen was without additives (control); groups 2 to 4: semen was diluted with 25 mM, 50 mM, and 100 mM of taurine, respectively. Seminal parameters and enzymatic and biochemical profiles were assessed at 5°C. Inclusion of taurine into diluent resulted in significant () decreases in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities after liquid storage compared with the control group. Additionally, taurine at 50 mM has significant improvement in quality of mithun semen than taurine at 25 or 100 mM stored in in vitro at 5°C. It was concluded that the possible protective effects of taurine on sperm parameters are from enhancing the function of antioxidant enzymes, preventing efflux of cholesterol from cell membranes and decreased MDA production. |
| format | Article |
| id | doaj-art-7edeab83280e431191e8b81e03f51eaa |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-7edeab83280e431191e8b81e03f51eaa2025-02-03T06:13:28ZengWileyVeterinary Medicine International2090-81132042-00482013-01-01201310.1155/2013/165348165348Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) SemenP. Perumal0Kezhavituo Vupru1C. Rajkhowa2Animal Reproduction Laboratory, National Research Centre on Mithun (ICAR), Jharnapani, Nagaland 797 106, IndiaAnimal Reproduction Laboratory, National Research Centre on Mithun (ICAR), Jharnapani, Nagaland 797 106, IndiaAnimal Reproduction Laboratory, National Research Centre on Mithun (ICAR), Jharnapani, Nagaland 797 106, IndiaThe present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliquots and diluted with the TEYC extender. Group 1: semen was without additives (control); groups 2 to 4: semen was diluted with 25 mM, 50 mM, and 100 mM of taurine, respectively. Seminal parameters and enzymatic and biochemical profiles were assessed at 5°C. Inclusion of taurine into diluent resulted in significant () decreases in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities after liquid storage compared with the control group. Additionally, taurine at 50 mM has significant improvement in quality of mithun semen than taurine at 25 or 100 mM stored in in vitro at 5°C. It was concluded that the possible protective effects of taurine on sperm parameters are from enhancing the function of antioxidant enzymes, preventing efflux of cholesterol from cell membranes and decreased MDA production.http://dx.doi.org/10.1155/2013/165348 |
| spellingShingle | P. Perumal Kezhavituo Vupru C. Rajkhowa Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen Veterinary Medicine International |
| title | Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen |
| title_full | Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen |
| title_fullStr | Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen |
| title_full_unstemmed | Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen |
| title_short | Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen |
| title_sort | effect of addition of taurine on the liquid storage 5°c of mithun bos frontalis semen |
| url | http://dx.doi.org/10.1155/2013/165348 |
| work_keys_str_mv | AT pperumal effectofadditionoftaurineontheliquidstorage5cofmithunbosfrontalissemen AT kezhavituovupru effectofadditionoftaurineontheliquidstorage5cofmithunbosfrontalissemen AT crajkhowa effectofadditionoftaurineontheliquidstorage5cofmithunbosfrontalissemen |