VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some spec...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/51 |
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| author | A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov |
| author_facet | A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov |
| author_sort | A. G. Bogomolov |
| collection | DOAJ |
| description | Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some species are not available. This article presents a new approach, which allows visualization of a signal from chromosome-specific DNAsequences by means of computer-assisted analysis of FISH images. |
| format | Article |
| id | doaj-art-7ebd0fe27638483c906cf10de4529efb |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-7ebd0fe27638483c906cf10de4529efb2025-02-01T09:57:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-0116234835735VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMESA. G. Bogomolov0K. S. Zadesenets1T. V. Karamysheva2N. L. Podkolodnyi3N. B. Rubtsov4Institute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia Institute of Computational Mathematics and Mathematical Geophysics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaPresently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some species are not available. This article presents a new approach, which allows visualization of a signal from chromosome-specific DNAsequences by means of computer-assisted analysis of FISH images.https://vavilov.elpub.ru/jour/article/view/51opisthorchis felineusmetorchis xanthosomushomo sapiensmeiotic chromosomesinterspersed repetitive dnachromosome-specific dna sequencesfluorescence in situ hybridization (fish)chromosomal in situ suppression hybridization (ciss-hybridization) |
| spellingShingle | A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES Вавиловский журнал генетики и селекции opisthorchis felineus metorchis xanthosomus homo sapiens meiotic chromosomes interspersed repetitive dna chromosome-specific dna sequences fluorescence in situ hybridization (fish) chromosomal in situ suppression hybridization (ciss-hybridization) |
| title | VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES |
| title_full | VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES |
| title_fullStr | VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES |
| title_full_unstemmed | VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES |
| title_short | VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES |
| title_sort | visualization of chromosome specific dna sequences by fluorescence in situ hybridization of microdissection dna probes with metaphase chromosomes |
| topic | opisthorchis felineus metorchis xanthosomus homo sapiens meiotic chromosomes interspersed repetitive dna chromosome-specific dna sequences fluorescence in situ hybridization (fish) chromosomal in situ suppression hybridization (ciss-hybridization) |
| url | https://vavilov.elpub.ru/jour/article/view/51 |
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