Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23)
Haemonchosis, caused by the abomasal nematode Haemonchus contortus, is a common parasitic disease of sheep. Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge. Recombinant DNA technology was applied to obtain a...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/852146 |
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| author | Leticia García-Coiradas Francisco Angulo-Cubillán Basilio Valladares Enrique Martínez Concepción de la Fuente José María Alunda Montserrat Cuquerella |
| author_facet | Leticia García-Coiradas Francisco Angulo-Cubillán Basilio Valladares Enrique Martínez Concepción de la Fuente José María Alunda Montserrat Cuquerella |
| author_sort | Leticia García-Coiradas |
| collection | DOAJ |
| description | Haemonchosis, caused by the abomasal nematode Haemonchus contortus, is a common parasitic disease of sheep. Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge. Recombinant DNA technology was applied to obtain a synthetic protein (rHcp26/23). Immunological assays (ELISA, Western blotting, and immunolocalization), using sera from lambs immunized with p26/23, confirmed the identity of the recombinant protein and demonstrated that the synthetic protein is equivalent to the purified protein employed in the previous immunoprophylaxis studies. Vaccination of lambs with 300 μg of rHcp26/23 and Freund's adjuvant elicited a notable specific antibody response. Immunization did not induce any significant protection after challenge with 16000 infective larvae of H. contortus, and comparable values for parasite faecal egg output, packed cell volume, and abomasal parasite burdens were found in vaccinated and control animals. |
| format | Article |
| id | doaj-art-7eb3bb3c38b34956afe8c6673b64c112 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-7eb3bb3c38b34956afe8c6673b64c1122025-02-03T05:54:17ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/852146852146Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23)Leticia García-Coiradas0Francisco Angulo-Cubillán1Basilio Valladares2Enrique Martínez3Concepción de la Fuente4José María Alunda5Montserrat Cuquerella6Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Enfermedades Transmisibles, Facultad de Veterinaria, Universidad del Zulia, 4005-A Maracaibo, VenezuelaDepartamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, 38271 Tenerife, SpainDepartamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, 38271 Tenerife, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainHaemonchosis, caused by the abomasal nematode Haemonchus contortus, is a common parasitic disease of sheep. Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge. Recombinant DNA technology was applied to obtain a synthetic protein (rHcp26/23). Immunological assays (ELISA, Western blotting, and immunolocalization), using sera from lambs immunized with p26/23, confirmed the identity of the recombinant protein and demonstrated that the synthetic protein is equivalent to the purified protein employed in the previous immunoprophylaxis studies. Vaccination of lambs with 300 μg of rHcp26/23 and Freund's adjuvant elicited a notable specific antibody response. Immunization did not induce any significant protection after challenge with 16000 infective larvae of H. contortus, and comparable values for parasite faecal egg output, packed cell volume, and abomasal parasite burdens were found in vaccinated and control animals.http://dx.doi.org/10.4061/2010/852146 |
| spellingShingle | Leticia García-Coiradas Francisco Angulo-Cubillán Basilio Valladares Enrique Martínez Concepción de la Fuente José María Alunda Montserrat Cuquerella Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) Veterinary Medicine International |
| title | Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) |
| title_full | Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) |
| title_fullStr | Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) |
| title_full_unstemmed | Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) |
| title_short | Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23) |
| title_sort | immunization against lamb haemonchosis with a recombinant somatic antigen of haemonchus contortus rhcp26 23 |
| url | http://dx.doi.org/10.4061/2010/852146 |
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