MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse
Nigrostriatal damage is increased in males relative to females. While estrogen is neuroprotective in females, less is known about potential protective effects of testosterone in males. We determined if castration enhances neuronal injury to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Castr...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.4061/2011/138471 |
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| author | Eleni Antzoulatos Michael W. Jakowec Giselle M. Petzinger Ruth I. Wood |
| author_facet | Eleni Antzoulatos Michael W. Jakowec Giselle M. Petzinger Ruth I. Wood |
| author_sort | Eleni Antzoulatos |
| collection | DOAJ |
| description | Nigrostriatal damage is increased in males relative to females. While estrogen is neuroprotective in females, less is known about potential protective effects of testosterone in males. We determined if castration enhances neuronal injury to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Castrates or sham-castrated mice were sacrificed 1 week following injection of MPTP (4×20 mg/kg) or saline (n=11-12/group). The right striatum was immunostained for tyrosine hydroxylase (TH). The left hemisphere was stained by Golgi Cox to quantify neuronal morphology in medium spiny neurons (MSNs) of the dorsolateral striatum. MPTP reduced TH, but there was no effect of castration and no interaction. For MSN dendritic morphology, MPTP decreased the highest branch order and increased spine density on 2nd-order dendrites. Castrated males had shorter 5th-order dendrites. However, there was no interaction between gonadal status and MPTP. Thus, castration and MPTP exert nonoverlapping effects on MSN morphology with castration acting on distal dendrites and MPTP acting proximally. |
| format | Article |
| id | doaj-art-7e89870553d44a07822f823973a2bfb5 |
| institution | Kabale University |
| issn | 2042-0080 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
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| series | Parkinson's Disease |
| spelling | doaj-art-7e89870553d44a07822f823973a2bfb52025-02-03T01:12:19ZengWileyParkinson's Disease2042-00802011-01-01201110.4061/2011/138471138471MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 MouseEleni Antzoulatos0Michael W. Jakowec1Giselle M. Petzinger2Ruth I. Wood3Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 90033, USADepartment of Neurology, Keck School of Medicine, Department of Biokinesiology, University of Southern California, Los Angeles, CA 90033, USADepartment of Neurology, Keck School of Medicine, Department of Biokinesiology, University of Southern California, Los Angeles, CA 90033, USADepartment of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USANigrostriatal damage is increased in males relative to females. While estrogen is neuroprotective in females, less is known about potential protective effects of testosterone in males. We determined if castration enhances neuronal injury to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Castrates or sham-castrated mice were sacrificed 1 week following injection of MPTP (4×20 mg/kg) or saline (n=11-12/group). The right striatum was immunostained for tyrosine hydroxylase (TH). The left hemisphere was stained by Golgi Cox to quantify neuronal morphology in medium spiny neurons (MSNs) of the dorsolateral striatum. MPTP reduced TH, but there was no effect of castration and no interaction. For MSN dendritic morphology, MPTP decreased the highest branch order and increased spine density on 2nd-order dendrites. Castrated males had shorter 5th-order dendrites. However, there was no interaction between gonadal status and MPTP. Thus, castration and MPTP exert nonoverlapping effects on MSN morphology with castration acting on distal dendrites and MPTP acting proximally.http://dx.doi.org/10.4061/2011/138471 |
| spellingShingle | Eleni Antzoulatos Michael W. Jakowec Giselle M. Petzinger Ruth I. Wood MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse Parkinson's Disease |
| title | MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse |
| title_full | MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse |
| title_fullStr | MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse |
| title_full_unstemmed | MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse |
| title_short | MPTP Neurotoxicity and Testosterone Induce Dendritic Remodeling of Striatal Medium Spiny Neurons in the C57Bl/6 Mouse |
| title_sort | mptp neurotoxicity and testosterone induce dendritic remodeling of striatal medium spiny neurons in the c57bl 6 mouse |
| url | http://dx.doi.org/10.4061/2011/138471 |
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