An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells
The study focuses on synthesizing, characterizing, and evaluating escin-loaded glutaraldehyde-albumin nanoparticles (NPs) against lung cancer A549 cells. The NPs were characterized using various methods, including UV–visible spectroscopy, photoluminescence, XRD, DLS, and TEM and SEM analyses. The cy...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625000049 |
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| author | Murugan Alwarkurichi Munusamy Muruganantham Bharathi Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Shobana Sampath Ali Kudumba |
| author_facet | Murugan Alwarkurichi Munusamy Muruganantham Bharathi Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Shobana Sampath Ali Kudumba |
| author_sort | Murugan Alwarkurichi Munusamy |
| collection | DOAJ |
| description | The study focuses on synthesizing, characterizing, and evaluating escin-loaded glutaraldehyde-albumin nanoparticles (NPs) against lung cancer A549 cells. The NPs were characterized using various methods, including UV–visible spectroscopy, photoluminescence, XRD, DLS, and TEM and SEM analyses. The cytotoxic effects of the NPs were evaluated using the WST-1 assay against A549 cells, and the expression of apoptotic proteins and oxidative stress markers was measured. The NPs were validated through FT-IR and DLS studies, and the WST-1 assay results showed that the synthesized albumin-escin NPs effectively inhibited A549 cells, induced apoptosis, and increased ATPase activity, and LDH enzyme release. The expression of apoptotic proteins and oxidative markers increased, while antiapoptotic protein and antioxidant levels were reduced in the albumin-Escin NPs-treated A549 cells. The study suggests that albumin-escin NPs have the potential as a promising anticancer agent against lung cancer by decreasing cell viability and promoting apoptosis. |
| format | Article |
| id | doaj-art-7e794dc3e1ee4275a81e70dacd74fe27 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-7e794dc3e1ee4275a81e70dacd74fe272025-01-29T05:00:59ZengElsevierResults in Chemistry2211-71562025-01-0113102021An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cellsMurugan Alwarkurichi Munusamy0Muruganantham Bharathi1Abdurahman Hajinur Hirad2Abdullah A. Alarfaj3Samer Hasan Hussein-Al-Ali4Shobana Sampath5Ali Kudumba6Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu 602 105, India; Corresponding authors.Centre for Bioinformatics, Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu 641021, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, P. O. Box.2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P. O. Box.2455, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, Faculty of Sciences, Isra University, Amman 11622, JordanDepartment of Biotechnology, Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai, Tamil Nadu, IndiaDepartment of Biological Sciences, Faculty of Science, Islamic University in Uganda, P.O. Box 2555 Mbale, Uganda; Corresponding authors.The study focuses on synthesizing, characterizing, and evaluating escin-loaded glutaraldehyde-albumin nanoparticles (NPs) against lung cancer A549 cells. The NPs were characterized using various methods, including UV–visible spectroscopy, photoluminescence, XRD, DLS, and TEM and SEM analyses. The cytotoxic effects of the NPs were evaluated using the WST-1 assay against A549 cells, and the expression of apoptotic proteins and oxidative stress markers was measured. The NPs were validated through FT-IR and DLS studies, and the WST-1 assay results showed that the synthesized albumin-escin NPs effectively inhibited A549 cells, induced apoptosis, and increased ATPase activity, and LDH enzyme release. The expression of apoptotic proteins and oxidative markers increased, while antiapoptotic protein and antioxidant levels were reduced in the albumin-Escin NPs-treated A549 cells. The study suggests that albumin-escin NPs have the potential as a promising anticancer agent against lung cancer by decreasing cell viability and promoting apoptosis.http://www.sciencedirect.com/science/article/pii/S2211715625000049NanoparticlesEscinApoptosisLung cancerOxidative stressAlbumin |
| spellingShingle | Murugan Alwarkurichi Munusamy Muruganantham Bharathi Abdurahman Hajinur Hirad Abdullah A. Alarfaj Samer Hasan Hussein-Al-Ali Shobana Sampath Ali Kudumba An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells Results in Chemistry Nanoparticles Escin Apoptosis Lung cancer Oxidative stress Albumin |
| title | An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells |
| title_full | An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells |
| title_fullStr | An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells |
| title_full_unstemmed | An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells |
| title_short | An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells |
| title_sort | escin loaded glutaraldehyde albumin nanoparticle system for enhancing anticancer activity on lung cancer a549 cells |
| topic | Nanoparticles Escin Apoptosis Lung cancer Oxidative stress Albumin |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625000049 |
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