Cloning and characterization of Bombyx mandarina serine protease-like protein
The complementary deoxyribonucleic acid (cDNA) of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 938 bp in length contained an open reading frame (ORF) of 912 bp which encoded 303 amino...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2014-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.12.251 |
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| Summary: | The complementary deoxyribonucleic acid (cDNA) of Bombyx mandarina serine protease-like protein gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 938 bp in length contained an open reading frame (ORF) of 912 bp which encoded 303 amino acid residues. The encoded amino acid sequence had a high identity to the reported sequence of serine protease-like protein from other insects and shared the typical structural features of serine protease family. The serine proteaselike protein gene was only expressed in haemolymph of B. mandarina by RT-PCR and Northern blot analysis. These results provide a molecular basis for further studying the function of this gene in B. mandarina. |
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| ISSN: | 1008-9209 2097-5155 |