Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins
BackgroundStaphylococcus aureus is a gram-positive bacterium commonly found in the nares and oropharynx of one in three individuals and has the potential to cause significant health problems. With antibiotic-resistant strains causing 11,000 deaths yearly and ~2% of the population nasally colonized w...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1457940/full |
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| author | Mark R. Dalman W. Brian Simison Danny Nielsen Sabana Bhatta Noor Ramahi Clair Yee Dipendra Thapaliya Jhalka Kadariya Shanice Cheatham Hailee Olson Tara C. Smith |
| author_facet | Mark R. Dalman W. Brian Simison Danny Nielsen Sabana Bhatta Noor Ramahi Clair Yee Dipendra Thapaliya Jhalka Kadariya Shanice Cheatham Hailee Olson Tara C. Smith |
| author_sort | Mark R. Dalman |
| collection | DOAJ |
| description | BackgroundStaphylococcus aureus is a gram-positive bacterium commonly found in the nares and oropharynx of one in three individuals and has the potential to cause significant health problems. With antibiotic-resistant strains causing 11,000 deaths yearly and ~2% of the population nasally colonized with methicillin-resistant S. aureus, a search for predictive markers and associative relationships between carriage have been long-sought goals. Within our study, we leveraged monozygotic twin participants in concert with multi-site microbiome analyses to characterize the impacts of S. aureus on composition.ResultsWe recruited 147 monozygotic twin pairs and characterized three sites, i.e., the nares, oropharynx, and hand microbiomes, using 16S rRNA v3-v4 sequencing in addition to S. aureus carriage status. The prevalence of S. aureus was highest in the oropharynx followed by nares and hand with concordance between twin pairs highest in the nares, followed by oropharynx. The detection of S. aureus was statistically correlated with differences in microbiome composition across sites, as indicated by beta diversity and DESeq2 analyses. Microbiome composition was most similar in twins’ nares that were S. aureus culture-positive concordant, whereas twins that were culture-negative concordant had the most similarity in the oropharynx. Of significance, Moraxella nonliquefacians and Capnocytophaga were inversely associated with S. aureus in the nares and oropharynx, respectively.ConclusionsThis improved understanding of S. aureus colonization in nares, oropharynx, and hand microbiomes in monozygotic twin pairs is a further step towards unraveling the degree to which the microbiome is influenced by host genetics and S. aureus carriage. |
| format | Article |
| id | doaj-art-7e35438fe51d49f09fe9ed5e97f0b335 |
| institution | Kabale University |
| issn | 2813-4338 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj-art-7e35438fe51d49f09fe9ed5e97f0b3352025-01-20T07:20:29ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382025-01-01310.3389/frmbi.2024.14579401457940Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twinsMark R. Dalman0W. Brian Simison1Danny Nielsen2Sabana Bhatta3Noor Ramahi4Clair Yee5Dipendra Thapaliya6Jhalka Kadariya7Shanice Cheatham8Hailee Olson9Tara C. Smith10College of Podiatric Medicine, Kent State University, Kent, OH, United StatesCenter for Comparative Genomics, California Academy of Sciences, San Francisco, CA, United StatesDepartment of Natural Resources and Environmental Science, University of Nevada, Reno, Reno, NV, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesCollege of Public Health, Kent State University, Kent, OH, United StatesBackgroundStaphylococcus aureus is a gram-positive bacterium commonly found in the nares and oropharynx of one in three individuals and has the potential to cause significant health problems. With antibiotic-resistant strains causing 11,000 deaths yearly and ~2% of the population nasally colonized with methicillin-resistant S. aureus, a search for predictive markers and associative relationships between carriage have been long-sought goals. Within our study, we leveraged monozygotic twin participants in concert with multi-site microbiome analyses to characterize the impacts of S. aureus on composition.ResultsWe recruited 147 monozygotic twin pairs and characterized three sites, i.e., the nares, oropharynx, and hand microbiomes, using 16S rRNA v3-v4 sequencing in addition to S. aureus carriage status. The prevalence of S. aureus was highest in the oropharynx followed by nares and hand with concordance between twin pairs highest in the nares, followed by oropharynx. The detection of S. aureus was statistically correlated with differences in microbiome composition across sites, as indicated by beta diversity and DESeq2 analyses. Microbiome composition was most similar in twins’ nares that were S. aureus culture-positive concordant, whereas twins that were culture-negative concordant had the most similarity in the oropharynx. Of significance, Moraxella nonliquefacians and Capnocytophaga were inversely associated with S. aureus in the nares and oropharynx, respectively.ConclusionsThis improved understanding of S. aureus colonization in nares, oropharynx, and hand microbiomes in monozygotic twin pairs is a further step towards unraveling the degree to which the microbiome is influenced by host genetics and S. aureus carriage.https://www.frontiersin.org/articles/10.3389/frmbi.2024.1457940/fullmicrobiome16S rRNAStaphylococcus aureusmonozygotic twinsnaresoropharynx |
| spellingShingle | Mark R. Dalman W. Brian Simison Danny Nielsen Sabana Bhatta Noor Ramahi Clair Yee Dipendra Thapaliya Jhalka Kadariya Shanice Cheatham Hailee Olson Tara C. Smith Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins Frontiers in Microbiomes microbiome 16S rRNA Staphylococcus aureus monozygotic twins nares oropharynx |
| title | Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins |
| title_full | Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins |
| title_fullStr | Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins |
| title_full_unstemmed | Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins |
| title_short | Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins |
| title_sort | staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx not the hand of monozygotic twins |
| topic | microbiome 16S rRNA Staphylococcus aureus monozygotic twins nares oropharynx |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1457940/full |
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