Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid
Papanicolaou staining is routinely used in staining ascitic fluid to identify malignant cells. However, pale nucleus arise as a problem associated with Harris hematoxylin, causing the sample to be hard to see under microscope. Methylene blue can be an alternative stain targeting nucleus in fluid sam...
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Poltekkes Kemenkes Jakarta III
2025-03-01
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| Series: | Jurnal Ilmu dan Teknologi Kesehatan |
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| Online Access: | https://ejurnal.poltekkesjakarta3.ac.id/index.php/jitek/article/view/1803 |
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| author | Acivrida Mega Charisma Melati Indah Permatasari Yohanes Ardian Kapri Negara |
| author_facet | Acivrida Mega Charisma Melati Indah Permatasari Yohanes Ardian Kapri Negara |
| author_sort | Acivrida Mega Charisma |
| collection | DOAJ |
| description | Papanicolaou staining is routinely used in staining ascitic fluid to identify malignant cells. However, pale nucleus arise as a problem associated with Harris hematoxylin, causing the sample to be hard to see under microscope. Methylene blue can be an alternative stain targeting nucleus in fluid sample. This study aims to evaluate the effectiveness of methylene blue in Papanicolaou staining for the cytology of ascitic fluid. The research uses an experimental approach by preparing four smears from each of 6 ascitic fluid samples obtained from the Laboratory of the Faculty of Medicine, at Airlangga University. The four smears were stained differently, consisting of Papanicolaou staining as control, and methylene blue replacing Harris hematoxylin with concentration of 5%, 10% and 15%. Slides were graded for its morphology with quality scores of 1,2,3 and 4, followed by statistical analysis. Based on the results of the Kruskal-Wallis test, the Sig value was 0.001, which was <0.005, showing difference between control and experimental group. Quality assessment revealed slides stained with 5% methylene blue solution to have clear shape of the cell nucleus, cytoplasm and the shape of the cell compared with slides stained with 10% and 15%. Hence, it can be concluded that 5% methylene blue solution can be used as an alternative solution. |
| format | Article |
| id | doaj-art-7e28b062eef64c8b9dcdde4eee1c8b9b |
| institution | DOAJ |
| issn | 2338-9095 2338-9109 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Poltekkes Kemenkes Jakarta III |
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| series | Jurnal Ilmu dan Teknologi Kesehatan |
| spelling | doaj-art-7e28b062eef64c8b9dcdde4eee1c8b9b2025-08-20T03:14:36ZengPoltekkes Kemenkes Jakarta IIIJurnal Ilmu dan Teknologi Kesehatan2338-90952338-91092025-03-01122175 – 181175 – 18110.32668/jitek.v12i2.18031803Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites FluidAcivrida Mega Charisma0Melati Indah Permatasari1Yohanes Ardian Kapri Negara2Universitas Anwar MedikaUniversitas Anwar MedikaUniversitas Anwar MedikaPapanicolaou staining is routinely used in staining ascitic fluid to identify malignant cells. However, pale nucleus arise as a problem associated with Harris hematoxylin, causing the sample to be hard to see under microscope. Methylene blue can be an alternative stain targeting nucleus in fluid sample. This study aims to evaluate the effectiveness of methylene blue in Papanicolaou staining for the cytology of ascitic fluid. The research uses an experimental approach by preparing four smears from each of 6 ascitic fluid samples obtained from the Laboratory of the Faculty of Medicine, at Airlangga University. The four smears were stained differently, consisting of Papanicolaou staining as control, and methylene blue replacing Harris hematoxylin with concentration of 5%, 10% and 15%. Slides were graded for its morphology with quality scores of 1,2,3 and 4, followed by statistical analysis. Based on the results of the Kruskal-Wallis test, the Sig value was 0.001, which was <0.005, showing difference between control and experimental group. Quality assessment revealed slides stained with 5% methylene blue solution to have clear shape of the cell nucleus, cytoplasm and the shape of the cell compared with slides stained with 10% and 15%. Hence, it can be concluded that 5% methylene blue solution can be used as an alternative solution.https://ejurnal.poltekkesjakarta3.ac.id/index.php/jitek/article/view/1803cytologyascitic fluidmethylene bluepapanicolaou staining |
| spellingShingle | Acivrida Mega Charisma Melati Indah Permatasari Yohanes Ardian Kapri Negara Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid Jurnal Ilmu dan Teknologi Kesehatan cytology ascitic fluid methylene blue papanicolaou staining |
| title | Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid |
| title_full | Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid |
| title_fullStr | Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid |
| title_full_unstemmed | Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid |
| title_short | Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid |
| title_sort | effectiveness of using methylene blue dyes in papanicolaou staining in cytology of ascites fluid |
| topic | cytology ascitic fluid methylene blue papanicolaou staining |
| url | https://ejurnal.poltekkesjakarta3.ac.id/index.php/jitek/article/view/1803 |
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