Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation
Galectin-1 (Gal-1) has immunomodulatory activities in various allergic inflammatory disorders, but its potential anti-inflammatory properties on allergic airway diseases have not been confirmed. We explored the pharmacological effects of Gal-1 on the progression of allergic airway inflammation and i...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/9705327 |
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| author | Yunxiang Lv Mengyuan Dai Muzi Wang Fangfang Chen Rongyu Liu |
| author_facet | Yunxiang Lv Mengyuan Dai Muzi Wang Fangfang Chen Rongyu Liu |
| author_sort | Yunxiang Lv |
| collection | DOAJ |
| description | Galectin-1 (Gal-1) has immunomodulatory activities in various allergic inflammatory disorders, but its potential anti-inflammatory properties on allergic airway diseases have not been confirmed. We explored the pharmacological effects of Gal-1 on the progression of allergic airway inflammation and investigated the underlying mechanism. Female C57BL/6 mice were sensitized on day 0 and challenged with ovalbumin (OVA) on days 14-17 to establish an allergic airway inflammation model. In the challenge phase, a subset of mice was treated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). We found that rGal-1 inhibited pulmonary inflammatory cell recruitment, mucus secretion, bronchoalveolar lavage fluid (BALF) inflammatory cell infiltration, and cytokine production. The treatment also suppressed the infiltration of eosinophils into the allergic lung as indicated by decreased expression levels of eotaxin and eosinophil peroxidase (EPX). However, only the expression levels of IL-25, neither IL-33 nor TSLP, were significantly decreased in the lung by rGal-1 treatment. These immunomodulatory effects in the allergic lung were correlated with the activation of extracellular signal-regulated kinase (ERK) signaling pathway and downregulation of endogenous Gal-1. In addition, rGal-1 reduced the plasma concentrations of anti-OVA immunoglobulin E (IgE) and IL-17. Our findings suggest that rGal-1 is an effective therapy for allergic airway inflammation in a murine model and may be a potential pharmacological target for allergic airway inflammatory diseases. |
| format | Article |
| id | doaj-art-7e04c0e61acd40eaa21feef70c7b9693 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7e04c0e61acd40eaa21feef70c7b96932025-02-03T06:04:56ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/97053279705327Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway InflammationYunxiang Lv0Mengyuan Dai1Muzi Wang2Fangfang Chen3Rongyu Liu4Department of Pulmonary Medicine, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei, Anhui 230022, ChinaDepartment of Pulmonary Medicine, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei, Anhui 230022, ChinaDepartment of Pulmonary Medicine, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei, Anhui 230022, ChinaDepartment of Pulmonary Medicine, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei, Anhui 230022, ChinaDepartment of Pulmonary Medicine, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei, Anhui 230022, ChinaGalectin-1 (Gal-1) has immunomodulatory activities in various allergic inflammatory disorders, but its potential anti-inflammatory properties on allergic airway diseases have not been confirmed. We explored the pharmacological effects of Gal-1 on the progression of allergic airway inflammation and investigated the underlying mechanism. Female C57BL/6 mice were sensitized on day 0 and challenged with ovalbumin (OVA) on days 14-17 to establish an allergic airway inflammation model. In the challenge phase, a subset of mice was treated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). We found that rGal-1 inhibited pulmonary inflammatory cell recruitment, mucus secretion, bronchoalveolar lavage fluid (BALF) inflammatory cell infiltration, and cytokine production. The treatment also suppressed the infiltration of eosinophils into the allergic lung as indicated by decreased expression levels of eotaxin and eosinophil peroxidase (EPX). However, only the expression levels of IL-25, neither IL-33 nor TSLP, were significantly decreased in the lung by rGal-1 treatment. These immunomodulatory effects in the allergic lung were correlated with the activation of extracellular signal-regulated kinase (ERK) signaling pathway and downregulation of endogenous Gal-1. In addition, rGal-1 reduced the plasma concentrations of anti-OVA immunoglobulin E (IgE) and IL-17. Our findings suggest that rGal-1 is an effective therapy for allergic airway inflammation in a murine model and may be a potential pharmacological target for allergic airway inflammatory diseases.http://dx.doi.org/10.1155/2019/9705327 |
| spellingShingle | Yunxiang Lv Mengyuan Dai Muzi Wang Fangfang Chen Rongyu Liu Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation Journal of Immunology Research |
| title | Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation |
| title_full | Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation |
| title_fullStr | Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation |
| title_full_unstemmed | Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation |
| title_short | Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation |
| title_sort | anti inflammatory property of galectin 1 in a murine model of allergic airway inflammation |
| url | http://dx.doi.org/10.1155/2019/9705327 |
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