Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran
Q fever is a zoonotic disease caused by Coxiella burnetii. This study investigated the prevalence of C. burnetii in traditional dairy products, specifically yogurt and cheese, in Lorestan Province. A total of 100 samples of traditional yogurt and unpasteurized cheese were collected from various regi...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Parasite Epidemiology and Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405673125000042 |
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| author | Soheila Mohammadkhanifard Amin Jaydari Ehsan Rashidian Nemat Shams Peyman Khademi |
| author_facet | Soheila Mohammadkhanifard Amin Jaydari Ehsan Rashidian Nemat Shams Peyman Khademi |
| author_sort | Soheila Mohammadkhanifard |
| collection | DOAJ |
| description | Q fever is a zoonotic disease caused by Coxiella burnetii. This study investigated the prevalence of C. burnetii in traditional dairy products, specifically yogurt and cheese, in Lorestan Province. A total of 100 samples of traditional yogurt and unpasteurized cheese were collected from various regions. To analyze the genome of C. burnetii, DNA was purified and molecular detection was performed using nested PCR with primers specific to the IS1111 transposon gene. The results revealed a prevalence of 13.3 % (95 % CI: 6.9 %–24.16 %) in yogurt samples and 12.5 % (95 % CI: 5.46 %–26.11 %) in cheese samples. Additionally, a significant seasonal variation in contamination levels was observed, with a p-value of less than 0.05. However, no significant correlation was found between geographical location and the degree of contamination. These findings suggest that the contamination of dairy products with C. burnetii is likely due to the bacterium's ‘spore-like’ form and the lack of pasteurization in the traditional production of yogurt and cheese. While the direct risk of transmission via unpasteurized dairy products is considered low, these products should still be monitored in Q fever outbreaks. |
| format | Article |
| id | doaj-art-7de9c27c972642c79e1b4c6b6a2155a8 |
| institution | Kabale University |
| issn | 2405-6731 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Parasite Epidemiology and Control |
| spelling | doaj-art-7de9c27c972642c79e1b4c6b6a2155a82025-02-02T05:27:44ZengElsevierParasite Epidemiology and Control2405-67312025-05-0129e00411Prevalence of Coxiella burnetii in unpasteurized dairy products in west of IranSoheila Mohammadkhanifard0Amin Jaydari1Ehsan Rashidian2Nemat Shams3Peyman Khademi4Department of Microbiology and Food Hygiene, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranCorresponding author at: Department of Microbiology and Health Food, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Lorestan, Iran.; Department of Microbiology and Food Hygiene, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranDepartment of Microbiology and Food Hygiene, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranDepartment of Microbiology and Food Hygiene, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranDepartment of Microbiology and Food Hygiene, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranQ fever is a zoonotic disease caused by Coxiella burnetii. This study investigated the prevalence of C. burnetii in traditional dairy products, specifically yogurt and cheese, in Lorestan Province. A total of 100 samples of traditional yogurt and unpasteurized cheese were collected from various regions. To analyze the genome of C. burnetii, DNA was purified and molecular detection was performed using nested PCR with primers specific to the IS1111 transposon gene. The results revealed a prevalence of 13.3 % (95 % CI: 6.9 %–24.16 %) in yogurt samples and 12.5 % (95 % CI: 5.46 %–26.11 %) in cheese samples. Additionally, a significant seasonal variation in contamination levels was observed, with a p-value of less than 0.05. However, no significant correlation was found between geographical location and the degree of contamination. These findings suggest that the contamination of dairy products with C. burnetii is likely due to the bacterium's ‘spore-like’ form and the lack of pasteurization in the traditional production of yogurt and cheese. While the direct risk of transmission via unpasteurized dairy products is considered low, these products should still be monitored in Q fever outbreaks.http://www.sciencedirect.com/science/article/pii/S2405673125000042Coxiella burnetiiNested-PCRDairy productsIS1111 gene |
| spellingShingle | Soheila Mohammadkhanifard Amin Jaydari Ehsan Rashidian Nemat Shams Peyman Khademi Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran Parasite Epidemiology and Control Coxiella burnetii Nested-PCR Dairy products IS1111 gene |
| title | Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran |
| title_full | Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran |
| title_fullStr | Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran |
| title_full_unstemmed | Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran |
| title_short | Prevalence of Coxiella burnetii in unpasteurized dairy products in west of Iran |
| title_sort | prevalence of coxiella burnetii in unpasteurized dairy products in west of iran |
| topic | Coxiella burnetii Nested-PCR Dairy products IS1111 gene |
| url | http://www.sciencedirect.com/science/article/pii/S2405673125000042 |
| work_keys_str_mv | AT soheilamohammadkhanifard prevalenceofcoxiellaburnetiiinunpasteurizeddairyproductsinwestofiran AT aminjaydari prevalenceofcoxiellaburnetiiinunpasteurizeddairyproductsinwestofiran AT ehsanrashidian prevalenceofcoxiellaburnetiiinunpasteurizeddairyproductsinwestofiran AT nematshams prevalenceofcoxiellaburnetiiinunpasteurizeddairyproductsinwestofiran AT peymankhademi prevalenceofcoxiellaburnetiiinunpasteurizeddairyproductsinwestofiran |