Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen
Interaction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNA vaccination has not been well understood. We examined whether administration of DNA encoding FasL gene enhanced ant...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/743828 |
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| author | Boya Zhong Guangyu Ma Ayako Sato Osamu Shimozato Hongdan Liu Quanhai Li Masato Shingyoji Yuji Tada Koichiro Tatsumi Hideaki Shimada Kenzo Hiroshima Masatoshi Tagawa |
| author_facet | Boya Zhong Guangyu Ma Ayako Sato Osamu Shimozato Hongdan Liu Quanhai Li Masato Shingyoji Yuji Tada Koichiro Tatsumi Hideaki Shimada Kenzo Hiroshima Masatoshi Tagawa |
| author_sort | Boya Zhong |
| collection | DOAJ |
| description | Interaction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNA
vaccination has not been well understood. We examined whether administration of DNA encoding FasL gene enhanced antitumor effects in mice that were vaccinated with DNA expressing a putative tumor antigen gene, β-galactosidase (β-gal). Growth of β-gal-positive Colon 26 tumors was retarded in the syngeneic mice immunized with β-gal and FasL DNA compared with those vaccinated with β-gal or FasL DNA. We did not detect increased numbers of β-gal-specific CD8+ T cells in lymph node of mice that received combination of β-gal and FasL DNA, but amounts of anti-β-gal antibody increased with the combination but not with β-gal or FasL DNA injection alone. Subtype analysis of anti-β-gal antibody produced by the combination of β-gal and FasL DNA or β-gal DNA injection showed that IgG2a amounts were greater in mice injected with both DNA than those with β-gal DNA alone, but IgG2b amounts were lower in both DNA-injected than β-gal DNA-injected mice. These data suggest that FasL is involved in boosting humoral immunity against a gene product encoded by coinjected DNA and enhances the vaccination effects. |
| format | Article |
| id | doaj-art-7dcaed6ba6ba47ef992fc40ad3086d0b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7dcaed6ba6ba47ef992fc40ad3086d0b2025-02-03T06:01:44ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/743828743828Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor AntigenBoya Zhong0Guangyu Ma1Ayako Sato2Osamu Shimozato3Hongdan Liu4Quanhai Li5Masato Shingyoji6Yuji Tada7Koichiro Tatsumi8Hideaki Shimada9Kenzo Hiroshima10Masatoshi Tagawa11Department of Hematology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, ChinaDepartment of Hematology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, ChinaDivision of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, JapanDivision of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, JapanDivision of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, JapanDivision of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, JapanDepartment of Thoracic Diseases, Chiba Cancer Center, Chiba 260-8717, JapanDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba 260-8670, JapanDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba 260-8670, JapanDepartment of Surgery, School of Medicine, Toho University, Tokyo 143-8541, JapanDepartment of Pathology, Tokyo Women’s Medical University Yachiyo Medical Center, Yachiyo 276-8524, JapanDivision of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, JapanInteraction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNA vaccination has not been well understood. We examined whether administration of DNA encoding FasL gene enhanced antitumor effects in mice that were vaccinated with DNA expressing a putative tumor antigen gene, β-galactosidase (β-gal). Growth of β-gal-positive Colon 26 tumors was retarded in the syngeneic mice immunized with β-gal and FasL DNA compared with those vaccinated with β-gal or FasL DNA. We did not detect increased numbers of β-gal-specific CD8+ T cells in lymph node of mice that received combination of β-gal and FasL DNA, but amounts of anti-β-gal antibody increased with the combination but not with β-gal or FasL DNA injection alone. Subtype analysis of anti-β-gal antibody produced by the combination of β-gal and FasL DNA or β-gal DNA injection showed that IgG2a amounts were greater in mice injected with both DNA than those with β-gal DNA alone, but IgG2b amounts were lower in both DNA-injected than β-gal DNA-injected mice. These data suggest that FasL is involved in boosting humoral immunity against a gene product encoded by coinjected DNA and enhances the vaccination effects.http://dx.doi.org/10.1155/2015/743828 |
| spellingShingle | Boya Zhong Guangyu Ma Ayako Sato Osamu Shimozato Hongdan Liu Quanhai Li Masato Shingyoji Yuji Tada Koichiro Tatsumi Hideaki Shimada Kenzo Hiroshima Masatoshi Tagawa Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen Journal of Immunology Research |
| title | Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen |
| title_full | Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen |
| title_fullStr | Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen |
| title_full_unstemmed | Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen |
| title_short | Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen |
| title_sort | fas ligand dna enhances a vaccination effect by coadministered dna encoding a tumor antigen through augmenting production of antibody against the tumor antigen |
| url | http://dx.doi.org/10.1155/2015/743828 |
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