The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits
Mesenchymal stem cells (MSCs) have shown chondroprotective effects in cartilage repair. However, side effects caused by MSC treatment limit their application in clinic. As a cell-free therapy, MSC-derived exosomes (EXOs) have attracted much more attention in recent years. In the present study, we pr...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/5760107 |
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| author | Hongwei Yang Meng Cong Weixiao Huang Jin Chen Min Zhang Xiaosong Gu Cheng Sun Huilin Yang |
| author_facet | Hongwei Yang Meng Cong Weixiao Huang Jin Chen Min Zhang Xiaosong Gu Cheng Sun Huilin Yang |
| author_sort | Hongwei Yang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) have shown chondroprotective effects in cartilage repair. However, side effects caused by MSC treatment limit their application in clinic. As a cell-free therapy, MSC-derived exosomes (EXOs) have attracted much more attention in recent years. In the present study, we prepared EXOs from human bone marrow mesenchymal stem cells (hBMSCs) and examined their therapeutic potentials in cartilage repair. Our results showed that the prepared extracellular vesicles exhibit classical features of EXOs, such as cup-like shape, around 100 nm diameter, positive protein markers (CD81, TSG101, and Flotillin 1), and ability of internalization. In primary chondrocytes, the treatment of hBMSC-EXOs markedly increases cell viability and proliferation in a dose-dependent manner. Moreover, wound healing assay showed that hBMSC-EXOs accelerate cell migration in primary chondrocytes. JC-1 staining revealed that the mitochondrial membrane potential was enhanced by hBMSC-EXOs, indicating cell apoptosis was decreased in the presence of hBMSC-EXOs. In rabbits with articular cartilage defects, local administration with hBMSC-EXOs facilitates cartilage regeneration as evidenced by gross view and hematoxylin-eosin (H&E) and Saf-O/Fast Green staining. In addition, the International Cartilage Repair Society (ICRS) score was increased by the application of hBMSC-EXOs. Overall, our data indicate that the treatment with hBMSC-EXOs is a suitable cell-free therapy for treating cartilage defects, and these benefits are likely due to improved cell proliferation and migration in chondrocytes. |
| format | Article |
| id | doaj-art-7dbb87cd0c0448179b55df72eacd4764 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7dbb87cd0c0448179b55df72eacd47642025-02-03T01:24:11ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/5760107The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in RabbitsHongwei Yang0Meng Cong1Weixiao Huang2Jin Chen3Min Zhang4Xiaosong Gu5Cheng Sun6Huilin Yang7Department of OrthopedicsKey Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Co-Innovation Center of NeuroregenerationKey Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Co-Innovation Center of NeuroregenerationDepartment of OrthopedicsKey Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Co-Innovation Center of NeuroregenerationKey Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Co-Innovation Center of NeuroregenerationKey Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Co-Innovation Center of NeuroregenerationDepartment of OrthopedicsMesenchymal stem cells (MSCs) have shown chondroprotective effects in cartilage repair. However, side effects caused by MSC treatment limit their application in clinic. As a cell-free therapy, MSC-derived exosomes (EXOs) have attracted much more attention in recent years. In the present study, we prepared EXOs from human bone marrow mesenchymal stem cells (hBMSCs) and examined their therapeutic potentials in cartilage repair. Our results showed that the prepared extracellular vesicles exhibit classical features of EXOs, such as cup-like shape, around 100 nm diameter, positive protein markers (CD81, TSG101, and Flotillin 1), and ability of internalization. In primary chondrocytes, the treatment of hBMSC-EXOs markedly increases cell viability and proliferation in a dose-dependent manner. Moreover, wound healing assay showed that hBMSC-EXOs accelerate cell migration in primary chondrocytes. JC-1 staining revealed that the mitochondrial membrane potential was enhanced by hBMSC-EXOs, indicating cell apoptosis was decreased in the presence of hBMSC-EXOs. In rabbits with articular cartilage defects, local administration with hBMSC-EXOs facilitates cartilage regeneration as evidenced by gross view and hematoxylin-eosin (H&E) and Saf-O/Fast Green staining. In addition, the International Cartilage Repair Society (ICRS) score was increased by the application of hBMSC-EXOs. Overall, our data indicate that the treatment with hBMSC-EXOs is a suitable cell-free therapy for treating cartilage defects, and these benefits are likely due to improved cell proliferation and migration in chondrocytes.http://dx.doi.org/10.1155/2022/5760107 |
| spellingShingle | Hongwei Yang Meng Cong Weixiao Huang Jin Chen Min Zhang Xiaosong Gu Cheng Sun Huilin Yang The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits Stem Cells International |
| title | The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits |
| title_full | The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits |
| title_fullStr | The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits |
| title_full_unstemmed | The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits |
| title_short | The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits |
| title_sort | effect of human bone marrow mesenchymal stem cell derived exosomes on cartilage repair in rabbits |
| url | http://dx.doi.org/10.1155/2022/5760107 |
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